Role of group A p21-activated kinases in activation of extracellular-regulated kinase by growth factors.
AuthorsBeeser A, Jaffer ZM, Hofmann C, Chernoff J,
JournalJ Biol Chem
PubMed ID16129686
'The canonical extracellular-regulated kinase (ERK) signaling cascade, consisting of the Ras-Raf-Mek-ERK module, is critically important to many cellular functions. Although the general mechanism of activation of the ERK cascade is well established, additional noncanonical components greatly influence the activity of this pathway. Here, we focus on the group A p21-activated ... More
p21-activated kinase 1 regulates microtubule dynamics by phosphorylating tubulin cofactor B.
AuthorsVadlamudi RK, Barnes CJ, Rayala S, Li F, Balasenthil S, Marcus S, Goodson HV, Sahin AA, Kumar R,
JournalMol Cell Biol
PubMed ID15831477
p21-activated kinase 1 (Pak1) induces cytoskeleton reorganization in part by regulating microtubule dynamics through an elusive mechanism. Using a yeast two-hybrid screen, we identified tubulin cofactor B (TCoB) (a cofactor in the assembly of the alpha/beta-tubulin heterodimers) as an interacting substrate of Pak1. Pak1 directly phosphorylated TCoB in vitro and ... More
Functional inactivation of a transcriptional corepressor by a signaling kinase.
The C-terminal binding protein 1 (CtBP) is a ubiquitous corepressor linking the recruitment of DNA- and histone-modifying proteins to sequence-specific DNA-binding proteins and facilitating gene regulation during development and oncogenesis. We describe here the binding, phosphorylation and functional regulation of CtBP by the p21-activated kinase 1 (Pak1). Pak1 phosphorylates CtBP ... More
Recent studies have identified dynein light chain-1 (DLC1), a component of the dynein motor, as a p21-activated kinase 1 (Pak1)-interacting substrate with binding sites mapped to amino acids 61-89 of DLC1 and phosphorylation site at serine 88. Here we investigated the role of DLC1 phosphorylation by Pak1 upon the process ... More
Regulation of phosphoglucomutase 1 phosphorylation and activity by a signaling kinase.
AuthorsGururaj A, Barnes CJ, Vadlamudi RK, Kumar R,
JournalOncogene
PubMed ID15378030
We have identified a novel mechanism of cross-talk between cell signaling and metabolic pathways, whereby the signaling kinase p21-activated kinase 1 (Pak1) binds to, phosphorylates and enhances the enzymatic activity of phosphoglucomutase 1 (PGM), an important regulatory enzyme in cellular glucose utilization and energy homeostasis. Pak1 and PGM were colocalized ... More
Filamin is essential in actin cytoskeletal assembly mediated by p21-activated kinase 1.
AuthorsVadlamudi RK, Li F, Adam L, Nguyen D, Ohta Y, Stossel TP, Kumar R,
JournalNat Cell Biol
PubMed ID12198493
The serine/threonine kinase p21-activated kinase 1 (Pak1) controls the actin cytoskeletal and ruffle formation through mechanisms that are independent of GTPase activity. Here we identify filamin FLNa as a Pak1-interacting protein through a yeast two-hybrid screen using the amino terminus of Pak1 as a bait. FLNa is stimulated by physiological ... More