ExoSAP-IT™ Express PCR 纯化产品

Name: ExoSAP-IT™ Express PCR 纯化产品
SKU: 75001.40.UL

只需 5 分钟，ExoSAP-IT™ Express PCR Product Cleanup Reagent 即可从 PCR了解更多信息

更改视图

货号	数量
75001.40.UL	40 μL
75001.4X.1.ML	2000 reactions
75001.1.EA	480 reactions
75001.10.ML	5000 reactions
75001.200.UL	100 reactions
75001.1.ML	500 reactions

6 选项

货号 75001.40.UL

价格（CNY)

数量:

40 μL

只需 5 分钟，ExoSAP-IT™ Express PCR Product Cleanup Reagent 即可从 PCR 反应中除去过量的引物和未结合的核苷酸。与替代纯化方法（如离心柱或微珠）相比，该酶法纯化方法是一种更精确、产量更高和周转时间更快的方法。

• Remove unincorporated primers and nucleotides in about 5 minutes
• Prepare DNA sequencing samples with one pipetting step
• Conserve precious PCR products with up to 100% recovery, regardless of amplicon length
• Minimize spin column or bead cleanup

ExoSAP-IT Express PCR Product Cleanup Reagent consists of two hydrolytic enzymes: a novel exonuclease I and shrimp alkaline phosphatase (SAP), in a specially formulated buffer. 反应设置仅需一个移液步骤即可完成，其后是两次短暂孵育。第一次孵育可以水解过量的引物并使核苷酸去磷酸化。第二次高温孵育在约一分钟内完全不可逆地灭活酶。将 ExoSAP-IT Express 试剂直接加入 PCR 产物中，可省略向管、孔或柱的转移步骤并节省 PCR 扩增子，从而有助于降低交叉污染的可能性；此外，也无需进一步处理。样品即可用于 DNA 测序或 SNP 分析。

仅供研究使用。不可用于诊断程序。

规格

反应次数20 Reactions

数量40 μL

测试时间5 min。

产品规格8联管, 96孔板, 单管

Unit SizeEach

内容与储存

置于干冰上运输。-20°C 保存。

常见问题解答 (FAQ)

Can ExoSAP-IT PCR cleanup reagents be used with automatic handling systems?

The HT ExoSAP-IT Fast High-Throughput PCR Product Cleanup reagent is designed for automated handlers as it has a lower viscosity than standard ExoSAP-IT PCR Product Cleanup reagent, which contains some glycerol.

After purification with ExoSAP-IT PCR cleanup reagents, what is the recommended quantification method?

The Quant-iT PicoGreen dsDNA Assay Kit is recommended for quantifying PCR products that have been cleaned with ExoSAP-IT reagent. ExoSAP-IT PCR cleanup results in 100% recovery regardless of amplicon length, and if the PCR product was quantified prior to ExoSAP-IT cleanup, additional quantification is not necessary. We do not recommend using a spectrophotometer as degraded products will be included in the quantification.

Can ExoSAP-IT PCR cleanup reagents be used for removal of primers after first-strand cDNA synthesis?

For use with reverse transcription, ExoSAP-IT does not degrade the DNA-RNA hybrid. However, it will degrade single-stranded cDNA if a 3' -OH end is present.

Will the two tracking dyes used for visualization in the DreamTaq Hot Start Green PCR Master Mix interfere with the ExoSAP-IT PCR cleanup reagents?

The tracking dyes used in the DreamTaq Hot Start Green PCR Master Mix have not been shown to interfere with functionality of the ExoSAP-IT cleanup reagents.

Can ExoSAP-IT PCR cleanup reagents be used to cleanup PCR products amplified using High Fidelity (HiFi) DNA polymerases?

The proofreading function of HiFi DNA polymerases can cause partial degradation of PCR products during extended incubation at 80°C. ExoSAP-IT Express reagent is recommended for HiFi PCR cleanup because it only requires 1 minute at 80°C for inactivation.

View all ExoSAP-IT™ Express PCR Product Cleanup FAQs