Rapid isolation of high-affinity protein binding peptides using bacterial display.
AuthorsBessette PH, Rice JJ, Daugherty PS
JournalProtein Eng Des Sel
PubMed ID15531628
'A robust bacterial display methodology was developed that allows the rapid isolation of peptides that bind to arbitrarily selected targets with high affinity. To demonstrate the utility of this approach, a large library (5 x 10(10) clones) was constructed composed of random 15-mer peptide insertions constrained within a flexible, surface ... More
Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein.
AuthorsShaner NC, Campbell RE, Steinbach PA, Giepmans BN, Palmer AE, Tsien RY
JournalNat Biotechnol
PubMed ID15558047
Fluorescent proteins are genetically encoded, easily imaged reporters crucial in biology and biotechnology. When a protein is tagged by fusion to a fluorescent protein, interactions between fluorescent proteins can undesirably disturb targeting or function. Unfortunately, all wild-type yellow-to-red fluorescent proteins reported so far are obligately tetrameric and often toxic or ... More