Halt™ 磷酸酶抑制剂单次使用混合物
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Halt™ 磷酸酶抑制剂单次使用混合物
引用和文献 (4)
Thermo Scientific™

Halt™ 磷酸酶抑制剂单次使用混合物

Thermo Scientific Halt 磷酸酶抑制剂单次使用混合物可在细胞裂解或组织蛋白提取期间和之后保持蛋白的磷酸化状态。Halt 磷酸酶抑制剂单次使用混合物的特点：•缺乏蛋白酶抑制剂—要保护蛋白免受蛋白酶和磷酸酶的影响，请使用 Halt 蛋白酶和磷酸酶抑制剂混合物。•透析—在执行与混合物成分不兼容的程序之前，可对样品进行透析或脱盐以有效去除细胞提取物中的抑制剂。•以了解更多信息
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货号数量
784201 mL
7842824 x 100μL
784265 x 1 mL
7842710 mL
货号 78420
价格（CNY)
2,320.00
飞享价
Ends: 31-Dec-2025
3,020.00
共减 700.00 (23%)
Each
添加至购物车
数量:
1 mL
请求批量或定制报价
价格（CNY)
2,320.00
飞享价
Ends: 31-Dec-2025
3,020.00
共减 700.00 (23%)
Each
添加至购物车
Thermo Scientific Halt 磷酸酶抑制剂单次使用混合物可在细胞裂解或组织蛋白提取期间和之后保持蛋白的磷酸化状态。

Halt 磷酸酶抑制剂单次使用混合物的特点：

•缺乏蛋白酶抑制剂—要保护蛋白免受蛋白酶和磷酸酶的影响，请使用 Halt 蛋白酶和磷酸酶抑制剂混合物。
•透析—在执行与混合物成分不兼容的程序之前，可对样品进行透析或脱盐以有效去除细胞提取物中的抑制剂。
•以 100X 浓缩物的形式提供—最终浓度为 1X 时也有效；然而，含有特别高水平磷酸酶活性的样品可能需要 2-3X 的最终浓度。
•兼容性—可与大多数含去污剂的细胞裂解试剂兼容，包括所有 Thermo Scientific Pierce 细胞裂解液。
•多合一规格 — 可抑制丝氨酸-苏氨酸磷酸酶和蛋白酪氨酸磷酸酶
•与常用蛋白测定兼容 — 可用于标准蛋白检测，包括 BCA 和 Coomassie (Bradford) 测定
•方便储存 — 在冷藏冰箱（2 至 8°C）中储存，可节省冷冻冰箱中的宝贵空间，并可立即使用

Halt 磷酸酶抑制剂混合物可在细胞裂解期间保护磷蛋白免受丝氨酸/苏氨酸磷酸酶以及蛋白酪氨酸磷酸酶 (PTP) 的影响。该混合物含四种广泛特异性抑制剂的混合物，包括氟化钠、原钒酸钠、焦磷酸钠和 β-磷酸甘油。以100倍形式共价包装，该混合物在4°C条件下稳定，并适合与任何细胞或组织抽提物配合使用。混合物可使蛋白在免疫沉淀和激酶试验中保持磷酸化。

磷酸化和去磷酸化包括调节细胞内许多关键生物通路的分子开关，包括信号转导、细胞分裂和细胞凋亡。为有效研究磷酸化活性状态，用户必须灭活磷酸酶以防止其在细胞裂解后的非受控活动。Halt 磷酸酶抑制剂混合物可达到这一目的。

相关产品
Pierce™ 磷酸酶抑制剂微型片剂
仅供科研使用。不可用于诊断程序。
规格
抑制剂磷酸酶抑制剂
数量1 mL
试剂类型磷酸酶抑制剂
形式液体
产品线Halt
产品类型抑制剂混合物
Unit SizeEach
内容与储存
接收后，在 4°C 下储存产品。切勿冷冻。

常见问题解答 (FAQ)

Do you offer the components of the Halt Protease and Phosphatase Cocktail individually?

Yes. Halt Protease, Halt Phosphatase and Protease/Phosphatase combined Inhibitor Cocktails are available. In addition, some of the individual inhibitors of the Halt Protease and Phosphatase Inhibitor Cocktail are offered separately.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Is the Halt Phosphatase Inhibitor Cocktail (Cat. No. 78420) EDTA-free?

Yes, the Halt Phosphatase Inhibitor Cocktail (Cat. No. 78420) is EDTA-free. It just contains the indicated phosphatase Inhibitors.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

I accidentally stored Halt Phosphatase Inhibitor Cocktail Bulk (100 mL) (Cat. No. 78420B) at -20 degrees C. Can I still use it?

We recommend storing Halt Phosphatase Inhibitor Cocktail Bulk (100 mL) at 4 degrees C. We do not recommend freezing it. One of the components of this cocktail (orthovandate) tends to form higher order structures and other oxidation states. These are colored products which can cause the cocktail to vary from colorless to yellow (and still be active). If the cocktail turns green, however, which can happen during freezing, it means that the orthovandate has lost its activity.

If the cocktail was accidentally frozen, we recommend thawing it out and examining the color. If the cocktail has turned green, we recommend discarding it.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

引用和文献 (4)

引用和文献
Abstract
Metabolic Alterations Caused by Simultaneous Loss of HK2 and PKM2 Leads to Photoreceptor Dysfunction and Degeneration.
Authors:Weh E,Goswami M,Chaudhury S,Fernando R,Miller N,Hager H,Sheskey S,Sharma V,Wubben TJ,Besirli CG
Journal:Cells
PubMed ID:37626853
HK2 and PKM2 are two main regulators of aerobic glycolysis. Photoreceptors (PRs) use aerobic glycolysis to produce the biomass necessary for the daily renewal of their outer segments. Previous work has shown that HK2 and PKM2 are important for the normal function and long-term survival of PRs but are dispensable ... More
DYRK2 controls GSTPI expression through ubiquitination and degradation of Twist1 to reduce chemotherapy resistance caused by EMT in breast cancer.
Authors:Xu G,Zhou M,Li Q,Li Y,Zhang C,Yang Z
Journal:Journal of molecular histology
PubMed ID:39641870
BACKGROUND: Breast cancer (BC) poses a significant global health challenge, with chemotherapy resistance, especially to docetaxel, remaining a major obstacle in effective treatment. The molecular mechanisms underlying this resistance are critical for developing targeted therapeutic strategies. OBJECTIVE: This study aims to explore the role of dual-specificity tyrosine phosphorylation-regulated kinase 2 ... More
Inhibition of Pim kinases triggers a broad antiviral activity by affecting innate immunity and via the PI3K-Akt-mTOR axis the endolysosomal system.
Authors:Glitscher M,Benz NI,Sabino C,Murra RO,Hein S,Zahn T,Mhedhbi I,Stefanova D,Bender D,Werner S,Hildt E
Journal:Antiviral research
PubMed ID:38649071
Zoonoses such as ZIKV and SARS-CoV-2 pose a severe risk to global health. There is urgent need for broad antiviral strategies based on host-targets filling gaps between pathogen emergence and availability of therapeutic or preventive strategies. Significant reduction of pathogen titers decreases spread of infections and thereby ensures health systems ... More
Tyrosine-Based Cross-Linking of Peptide Antigens to Generate Nanoclusters with Enhanced Immunogenicity: Demonstration Using the Conserved M2e Peptide of Influenza A.
Authors:Wilks LR,Joshi G,Grisham MR,Gill HS
Journal:ACS infectious diseases
PubMed ID:34432416
A method of creating nanoclusters (NCs) from soluble peptide molecules is described utilizing an approach based on a tyrosine-tyrosine cross-linking reaction. A reactive tag comprising histidine and tyrosine residues was introduced at the termini of the peptide molecules. The cross-linking reaction led to the creation of dityrosine bonds within the ... More