Modification of histidine repeat proteins by inorganic polyphosphate.
AuthorsNeville N,Lehotsky K,Yang Z,Klupt KA,Denoncourt A,Downey M,Jia Z
JournalCell reports
PubMed ID37660293
Inorganic polyphosphate (polyP) is a linear polymer of orthophosphate that is present in nearly all organisms studied to date. A remarkable function of polyP involves its attachment to lysine residues via non-enzymatic post-translational modification (PTM), which is presumed to be covalent. Here, we show that proteins containing tracts of consecutive ... More
Deletion of the prorenin receptor in the ureteric bud in mice inhibits Dot1/H3K79 pathway.
AuthorsSong R,Yosypiv IV
JournalPediatric research
PubMed ID38287105
BACKGROUND: The prorenin receptor (PRR) plays a critical role in ureteric bud (UB) branching morphogenesis. DOT1 Like (DOT1L), a histone methyltransferase specific for Histone 3 lysine 79 (H3K79), is important for differentiation of the UB-derived renal collecting duct cells. In this study, we tested whether DOT1L/H3 dimethyl K79 (H3m2K79) are ... More
Metformin inhibits digestive proteases and impairs protein digestion in mice.
AuthorsKelly CJ,Verdegaal AA,Anderson BW,Shaw WL,Bencivenga-Barry NA,Folta-Stogniew E,Goodman AL
JournalThe Journal of biological chemistry
PubMed ID37863262
Metformin is among the most prescribed medications worldwide and the first-line therapy for type 2 diabetes. However, gastrointestinal side effects are common and can be dose limiting. The total daily metformin dose frequently reaches several grams, and poor absorption results in high intestinal drug concentrations. Here, we report that metformin ... More
β-Cell-specific ablation of sirtuin 4 does not affect nutrient-stimulated insulin secretion in mice.
JournalAmerican journal of physiology. Endocrinology and metabolism
PubMed ID32865009
Sirtuins are a family of proteins that regulate biological processes such as cellular stress and aging by removing posttranslational modifications (PTMs). We recently identified several novel PTMs that can be removed by sirtuin 4 (SIRT4), which is found in mitochondria. We showed that mice with a global loss of SIRT4 ... More
Glucagon-like peptide 1 inhibits cell apoptosis and improves glucose responsiveness of freshly isolated human islets.
AuthorsFarilla L, Bulotta A, Hirshberg B, Li Calzi S, Khoury N, Noushmehr H, Bertolotto C, Di Mario U, Harlan DM, Perfetti R
JournalEndocrinology
PubMed ID12960095
The peptide hormone, glucagon-like peptide 1 (GLP-1), has been shown to increase glucose-dependent insulin secretion, enhance insulin gene transcription, expand islet cell mass, and inhibit beta-cell apoptosis in animal models of diabetes. The aim of the present study was to evaluate whether GLP-1 could improve function and inhibit apoptosis in ... More