Halt™ Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X), 1 mL

Halt™ Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X), 1 mL - FAQs

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6 个常见问题解答

Can I store the 1X solution of the Halt Protease and Phosphatase Inhbitor Cocktail and if so, for how long?

The stability of the 1X solution is undetermined. For best results, prepare solutions just before use. The 100X concentrate is stable for at least one year stored at 4 degrees C.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What are the concentrations of the various components of the combined Halt Protease and Phosphatase Inhibitor Cocktail?

Although we reveal the identity of the inhibitors in the combined cocktail, their concentrations are proprietary.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Do you offer the components of the Halt Protease and Phosphatase Cocktail individually?

Yes. Halt Protease, Halt Phosphatase and Protease/Phosphatase combined Inhibitor Cocktails are available. In addition, some of the individual inhibitors of the Halt Protease and Phosphatase Inhibitor Cocktail are offered separately.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

When do I need to add EDTA when using the Halt Protease and Phosphatase Cocktail?

EDTA chelates the metal ions required for metalloprotease activity. Add EDTA if metalloproteases are likely to adversely affect target protein function; however, avoid using EDTA when the downstream application involves metal chelate affinity purification or the target protein requires metals for proper function.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

How much of the Halt Protease and Phosphatase Cocktail do I use for my sample?

The Halt Protease and Phosphatase Cocktail is supplied at a 100X concentrate and is used at 1X. For example, add 10µL of 100X Halt Cocktail to 1mL of sample. Some samples, however, contain high levels of proteases or phosphatase and might require a more concentrated treatment (i.e., 2-3X).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What formats are available for the combined Halt Protease and Phosphatase Inhibitor Cocktails?

The Halt Protease and Phosphatase Inhibitor Cocktails are offered as 100X concentrates in single-use or multiple-use formats. The metalloprotease inhibitor, EDTA, is provided in separate vials.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.