Impact of carbamazepine on SMARCA4 (BRG1) expression in colorectal cancer: modulation by KRAS mutation status.
AuthorsShaykevich A,Chae D,Silverman I,Bassali J,Louloueian N,Siegman A,Bandyopadhyaya G,Goel S,Maitra R
JournalInvestigational new drugs
PubMed ID38446332
SMARCA4 is a gene traditionally considered a tumor suppressor. Recent research has however found that SMARCA4 likely promotes cancer growth and is a good target for cancer treatment. The drug carbamazepine, an autophagy inducer, was used on colorectal cancer cell lines, HCT1116 and Hke3 (KRAS mutant and wildtype). Our study ... More
(3'R)-hydroxytabernaelegantine C: A bisindole alkaloid with potent apoptosis inducing activity in colon (HCT116, SW620) and liver (HepG2) cancer cells.
AuthorsPaterna A,Gomes SE,Borralho PM,Mulhovo S,Rodrigues CM,Ferreira MU
JournalJournal of ethnopharmacology
PubMed ID27616029
ETHNOPHARMACOLOGICAL RELEVANCE: Tabernaemontana elegans Stapf. (Apocynaceae) is a medicinal plant traditionally used in African countries to treat cancer. AIMS OF THE STUDY: To discover new apoptosis inducing lead compounds from T. elegans and provide scientific validation of the ethnopharmacological use of this plant. MATERIALS AND METHODS: Through fractionation, (3'R)-hydroxytaberanelegantine C ... More
AuthorsMansoor TA, Borralho PM, Luo X, Mulhovo S, Rodrigues CM, Ferreira MJ.
JournalJ Nat Prod
PubMed ID25066282
6-Acetonyldihydrochelerythrine (1), a benzophenanthridine alkaloid, isolated from the methanol extract of Zanthoxylum capense, displayed potent cytotoxic activity in human HCT116 and SW620 colon carcinoma cells, to a higher extent than 5-fluorouracil (5-FU), the cornerstone chemotherapeutic agent in colon cancer. Cytotoxicity of 1 was evaluated by MTS, lactate dehydrogenase (LDH), and ... More
CDK4/6 activity is required during G(2) arrest to prevent stress-induced endoreplication.
AuthorsMcKenney C,Lendner Y,Guerrero Zuniga A,Sinha N,Veresko B,Aikin TJ,Regot S
JournalScience (New York, N.Y.)
PubMed ID38696576
Cell cycle events are coordinated by cyclin-dependent kinases (CDKs) to ensure robust cell division. CDK4/6 and CDK2 regulate the growth 1 (G(1)) to synthesis (S) phase transition of the cell cycle by responding to mitogen signaling, promoting E2F transcription and inhibition of the anaphase-promoting complex. We found that this mechanism ... More
Quantitative PCR analysis of DNA, RNAs, and proteins in the same single cell.
AuthorsStåhlberg A, Thomsen C, Ruff D, Åman P
JournalClin Chem
PubMed ID23014600
The single cell represents the basic unit of all organisms. Most investigations have been performed on large cell populations, but understanding cell dynamics and heterogeneity requires single-cell analysis. Current methods for single-cell analysis generally can detect only one class of analytes. ... More
The Src inhibitor dasatinib accelerates the differentiation of human bone marrow-derived mesenchymal stromal cells into osteoblasts.
AuthorsId Boufker H, Lagneaux L, Najar M, Piccart M, Ghanem G, Body JJ, Journé F
JournalBMC Cancer
PubMed ID20565769
The proto-oncogene Src is an important non-receptor protein tyrosine kinase involved in signaling pathways that control cell adhesion, growth, migration and differentiation. It negatively regulates osteoblast activity, and, as such, its inhibition is a potential means to prevent bone loss. Dasatinib is a new dual Src/Bcr-Abl tyrosine kinase inhibitor initially ... More