Forkhead box O1 (FOXO1) is upregulated during bone formation and in response to stimulation by bone morphogenetic proteins. Studies presented here examined the functional role of FOXO1 in a well defined culture system in which pre-osteoblastic cells undergo terminal differentiation in vitro. Mineralizing cultures of MC3T3-E1 cells were examined with ... More
Correlation between S100A11 and the TGF-β(1)/SMAD4 pathway and its effects on the proliferation and apoptosis of pancreatic cancer cell line PANC-1.
S100A11 as a S100 protein family member has been documented to play dual-direction regulation over cancer cell proliferation. We explored the role of S100A11 in the proliferation and apoptosis of pancreatic cancer cell line PANC-1 and the potential mechanisms involving the TGF-β(1)/SMAD4/p21 pathway. S100A11 and TGF-β(1) protein expressions in 30 ... More
An improved cell nuclear isolation method.
AuthorsLi P,Zhang J,Liu X,Wu Z,Kang YJ,Zhang W
JournalBiology methods & protocols
PubMed ID39925781
Nuclear isolation is crucial for studying gene expression and regulatory mechanisms in eukaryotic cells. This study aimed to improve nuclear isolation and compare the yield, purity, and efficiency of several methods. Human umbilical vein endothelial cells were used to evaluate four different techniques: sucrose centrifugation, a simplified method, homogenization, and ... More
Conformation-Crooking CXCL4 to Unravel Autoimmune Heparin-Induced Thrombocytopenia.
AuthorsKoenen RR
JournalThrombosis and haemostasis
PubMed ID33385179
Human bile salt export pump promoter is transactivated by the farnesoid X receptor/bile acid receptor.
AuthorsAnanthanarayanan M, Balasubramanian N, Makishima M, Mangelsdorf DJ, Suchy FJ
JournalJ Biol Chem
PubMed ID11387316
'The bile salt excretory pump (BSEP, ABCb11) is critical for ATP-dependent transport of bile acids across the hepatocyte canalicular membrane and for generation of bile acid-dependent bile secretion. Recent studies have demonstrated that the expression of this transporter is sensitive to the flux of bile acids through the hepatocyte, possibly ... More
Radiation-induced epidermal growth factor receptor nuclear import is linked to activation of DNA-dependent protein kinase.
AuthorsDittmann K, Mayer C, Fehrenbacher B, Schaller M, Raju U, Milas L, Chen DJ, Kehlbach R, Rodemann HP
JournalJ Biol Chem
PubMed ID16000298
Ionizing radiation, but not stimulation with epidermal growth factor (EGF), triggers EGF receptor (EGFR) import into the nucleus in a probably karyopherin alpha-linked manner. An increase in nuclear EGFR is also observed after treatment with H2O2, heat, or cisplatin. During, this process, the proteins Ku70/80 and the protein phosphatase 1 ... More
Curcumin interrupts the interaction between the androgen receptor and Wnt/ß-catenin signaling pathway in LNCaP prostate cancer cells.
AuthorsChoi HY, Lim JE, Hong JH
JournalProstate Cancer Prostatic Dis
PubMed ID20680030
Recently, studies have investigated the significance of the Wnt/ß-catenin pathway in prostate cancer. The transcriptional activity of the androgen receptor (AR) is modulated by interaction with coregulators, one of which is ß-catenin. Curcumin, a dietary yellow pigment of Curcuma longa, has emerged as having a chemopreventive role. Although curcumin has ... More