DRIM modulates Src activation and regulates angiogenic functions in vascular endothelial cells.
AuthorsTong J,Dong X,Martin TA,Yang Y,Dong B,Jiang WG
JournalCell biology international
PubMed ID39648301
Downregulated in Metastasis Protein (DRIM) was discovered in malignant epithelial cells and was thought to be mainly a nucleus protein affecting cancer cells. Recent single-cell sequencing analysis suggests that DRIM is abundantly expressed in vascular endothelial cells. There has been no knowledge of the role of DRIM in the endothelium. ... More
Protocol to identify human subcellular alternative protein interactions using cross-linking mass spectrometry.
AuthorsGarcia-Del Rio DF,Fournier I,Cardon T,Salzet M
JournalSTAR protocols
PubMed ID37384523
Since the start of mass-spectrometry-based proteomics, proteins from non-referenced open reading frames or alternative proteins (AltProts) have been overlooked. Here, we present a protocol to identify human subcellular AltProt and decipher some interactions using cross-linking mass spectrometry. We describe steps for cell culture, in cellulo cross-link, subcellular extraction, and sequential digestion. ... More
Evaluation of a Low-Toxicity PARP Inhibitor as a Neuroprotective Agent for Parkinson's Disease.
Repurposing PARP-1 inhibitors (PARPi) for non-oncological applications offers an attractive therapeutic strategy for pathological conditions characterized by PARP-1 hyperactivity. In the context of Parkinson's disease (PD), PARP-1 hyperactivity has been linked to neuronal death and disease progression. From a therapy perspective, the evaluation of PARPi as neuroprotective agents may offer ... More