DynaGreen™ Protein A/G 磁珠
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DynaGreen™ Protein A/G 磁珠
Invitrogen™

DynaGreen™ Protein A/G 磁珠

Green features
DynaGreen Protein A/G 磁珠能够实现高性能直接和间接法免疫沉淀,同时也是一种环境可持续的选择。DynaGreen Protein A/G 磁珠是不含微塑料的亚微米级磁珠。磁珠可在简单高效的工作流程中提供可重现的靶蛋白分离,可支持手动方案或在 KingFisher了解更多信息
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货号数量
80105G3 mL
80104G0.5 mL
80106G25 mL
货号 80105G
价格(CNY)
4,792.00
飞享价
Ends: 31-Dec-2025
5,990.00
共减 1,198.00 (20%)
Each
添加至购物车
数量:
3 mL
价格(CNY)
4,792.00
飞享价
Ends: 31-Dec-2025
5,990.00
共减 1,198.00 (20%)
Each
添加至购物车
DynaGreen Protein A/G 磁珠能够实现高性能直接和间接法免疫沉淀,同时也是一种环境可持续的选择。DynaGreen Protein A/G 磁珠是不含微塑料的亚微米级磁珠。磁珠可在简单高效的工作流程中提供可重现的靶蛋白分离,可支持手动方案或在 KingFisher 仪器上实现自动化。

特点包括:
•产生可重现的高产量和高质量的免疫沉淀
• 减少研究对环境的影响
• 提高纯度,降低非特异性结合
• 轻松实现扩展和自动化操作
• 无孔磁珠,减少抗体用量

手动分离简单、温和、高效
DynaGreen Protein A/G 磁珠是不含微塑料的超顺磁珠,表面共价偶联重组 Protein A/G。亚微米级磁珠粒径较小(约 250 nm),降低了沉降速率,增加了靶标捕获表面积。因此在无需预清洗步骤的简单直接或间接法免疫沉淀(结合 - 孵育 - 洗脱)方案中,80 分钟内即可实现靶蛋白的高效和高产量分离。

DynaGreen Protein A/G 磁珠使用的磁性分离技术快速而温和,对靶蛋白产生的物理压力非常小。此外,磁珠表面的无孔特性意味着抗体卡住导致无法接近的风险较低,最大限度地减少了产生准确和可重复结果所需的抗体量。所分离的高产量和高纯度的靶蛋白与下游免疫印迹或质谱高度兼容。

以下产品手册包括所需试剂和材料的清单以及兼容的缓冲液配方的详细信息。

快速可重现的自动化工作流程
随着实验规模增加到中高通量,DynaGreen Protein A/G 磁珠的工作流程可以使用 KingFisher 自动化平台无缝扩展。自动化方案仅需 40 分钟即可运行完成,每次处理高达 96 个样品,大大减少了手动操作时间,同时保持与手动方案相同的可重现的高靶标产量和低非特异性结合。

环境可持续研究的认证选择
不含微塑料的 DynaGreen Protein A/G 磁珠在设计时既考虑了提高免疫沉淀工作流程的性能,也考虑了环境因素。与其他磁珠替代产品相比,在直接和间接法免疫沉淀工作流程中,已经证明不含微塑料的 DynaGreen Protein A/G 磁珠能够提供高纯度和高产量的靶蛋白。同时,该产品通过结合绿色化学和绿色工程的原则,包含大量第三方 ACT 标签认证的可持续产品设计元素。选择 DynaGreen Protein A/G 磁珠有助于在不影响研究结果的情况下减少研究对环境的影响。

仅供科研使用。不可用于诊断程序。
规格
认证/合规ISO9001 和 ISO13485
颜色棕色
最大浓度20 mg/mL
描述Recombinant Protein A/G covalently bound to DynaGreen magnetic beads
直径(公制)250 nm
适用于(应用)免疫沉淀
适用于(设备)DynaMag 磁力架或 KingFisher 仪器
环保功能采用环保包装,降低危害,减少废弃物
高通量能力兼容高通量应用
配基类型重组Protein A/G
材料氧化铁
纯度或质量等级For Research Use Only
数量3 mL
有效期12 个月
运输条件室温
足够用于120 Tests
靶标Proteins
产品线DynaGreen™
类型磁珠
Unit SizeEach
内容与储存
20 mg/mL 磁珠溶于 pH 值为 7.4 的磷酸盐缓冲溶液(PBS)中,以及生物降解表面活性剂和防腐剂。

常见问题解答 (FAQ)

What can I do if the antibody from the DynaGreen magnetic beads is coming off the beads during the elution step?

The antibody will come off the beads during elution if the antibody is not cross-linked to the beads. The antibody is attached to the Protein A/G through affinity binding, thus when you break one of the affinity bindings, the other affinity bindings will break as well.

Find additional tips, troubleshooting help, and resources within our Protein Immunoprecipitation (IP), Co-Immunoprecipitation (Co-IP), and Pulldown Support Center.

Do you have any tips to offer if we are experiencing non-specific protein binding to DynaGreen magnetic beads or proteins trapped in the protein-antibody-antigen immunocomplex?

Here are some tips:
- Perform a pre-clearing procedure before doing immunoprecipitation (IP) by adding the beads to the sample without the antibody bound. After this pre-clearing step, continue with the IP protocol using the antibody-bound beads.
- Increase the washing time and increase the number of washing steps after IP.
- Increase the stringency of the washes, e.g., wash with a buffer containing a higher concentration of detergent or salt.
- Try the indirect IP technique (add antibody to the sample first, and then add the beads), change to another DynaGreen product (e.g., switch from DynaGreen Protein A/G Magnetic Beads to DynaGreen CaptureSelect Anti IgG-Fc Magnetic Beads), or try Dynabeads Protein A or Dynabeads Protein G for Immunoprecipitation.

Find additional tips, troubleshooting help, and resources within our Protein Immunoprecipitation (IP), Co-Immunoprecipitation (Co-IP), and Pulldown Support Center.

When using DynaGreen magnetic beads, how can we avoid sample degradation by proteases?

We recommend including additional protease inhibitors in the lysis and wash buffers and keeping the sample cold at all times.

Find additional tips, troubleshooting help, and resources within our Protein Immunoprecipitation (IP), Co-Immunoprecipitation (Co-IP), and Pulldown Support Center.

How can I improve the elution of the target from DynaGreen magnetic beads using a low pH buffer?

The addition of a small amount of detergent (eg., 0.05% of Tween 20) in the wash buffer, before elution, can improve the elution of the target. Also, it is important to fully remove the wash buffer before adding the elution buffer, in order to maintain the low pH conditions required for elution.

Find additional tips, troubleshooting help, and resources within our Protein Immunoprecipitation (IP), Co-Immunoprecipitation (Co-IP), and Pulldown Support Center.

Are DynaGreen magnetic beads sterile?

Per definition, DynaGreen magnetic beads are not sterile, but all the buffers used in the manufacturing process are autoclaved and a biocide called Acticide is added to the product to help keep it free of any type of growth.

Find additional tips, troubleshooting help, and resources within our Protein Immunoprecipitation (IP), Co-Immunoprecipitation (Co-IP), and Pulldown Support Center.