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查看更多产品信息 815025DE - FAQs (815025DE)
20 个常见问题解答
试剂盒和定制服务有不同的优点。请点击此处（https://www.thermofisher.com/us/en/home/life-science/cloning/gene-synthesis/geneart-gene-synthesis-product-selection-guide.html）查看一份对技术，递送和生产时间进行比较的选择指南，以决定哪种最适合您。
GeneArt Strings DNA片段是寡核苷酸组装后进行的PCR扩增子，可直接用于筛选正确克隆。作为质量控制（QC）过程的一部分，我们对Strings片段进行整体序列控制（bulk sequence-controlled）以确保您需要的序列包含在片段库中。
To identify a correct clone >为了使正确克隆的获得率>90%，我们建议您使用以下这些筛选指南：
•小于1 kb的Strings片段：测序2-4个全长克隆
•1-2 kb的Strings片段：测序3-5个全长克隆
•2-3 kb的Strings片段：测序4-8个全长克隆
不是，GeneArt Strings DNA片段仅能合成100至3000 bp范围内的片段。如果您的序列长度超过3000 bp，则需要订购基因合成服务或通过2个或更多个GeneArt Strings DNA片段组装您的基因。另外，GeneArt Strings DNA片段流水线生产，可以快速、经济地为您提供基因产品。如果您的序列比较复杂，可能会收到一条信息，告诉您Strings DNA片段由于序列的高度复杂性而不能生产。在这种情况下，我们建议修改您的序列以满足生产标准（后文给出）或使用基因合成服务合成您的基因。在很多情况下，使用门户网站功能优化您的序列即可满足GeneArt Strings片段的生产要求。如果仍然不能生产，您可以手动编辑序列以满足生产要求。
重要的生产标准有：
•GC含量20至80%（仅指峰值GC含量，而非总含量）
•没有长的二级结构或重复序列
•没有>25 bp的A/T延伸结构，也没有>20 bp的G/C延伸结构
可以，可以直接将两个GeneArt Strings片段组装起来，无需预克隆。我们提供好几种无缝组装技术，例如，GeneArt Type IIs组装技术或GeneArt Seamless克隆和组装技术。根据序列长度和您想要组装的片段数的不同，筛选阳性克隆的难度可能会很高。因此，我们通常推荐对亚片段进行预克隆以降低在组装后筛选阳性克隆的难度。
不提供，GeneArt Strings DNA 片段不提供亚克隆或组装服务，因为其服务设计理念是为您提供获得基因的最快、最经济的方式。如果您不想自己克隆基因，我们建议您订购全基因合成服务。
是的。我们建议使用GeneArt网络门户进行订购，在这里您可以使用免费的基因编辑和优化功能修改序列以满足您的需求。在编辑和优化之后，请再次检查确认您的最终序列，确认其准确满足您后续实验需求（例如，片段可能的同源重叠或限制性内切酶位点以及克隆所需的缓冲液）。
不同。100至1000bp的GeneArt Strings DNA片段生产需要5个工作日，1000至3000bp的GeneArt Strings DNA片段生产需要8个工作日。根据序列的性质不同，生产时间也会不同。
GeneArt Strings DNA片段以干粉形式运输，可直接进行重悬和克隆。在使用前，我们建议将试管内的粉末离心至管底，向试管的底部加水以获得想要的浓度，然后室温孵育至少1小时（也可4摄氏度下孵育过夜）。接下来，我们建议小心重悬片段并立即使用。如需长期储存，重悬后的GeneArt Strings DNA片段应分装并冻存在-20摄氏度。请避免反复冻融。
GeneArt Strings DNA片段是组装好的寡核苷酸进行PCR得到的扩增子；是基因合成过程的一个中间产物。这一过程形成一个片段库，并且需要进行克隆和筛选以找到正确的克隆。为确保片段库中含有正确的片段，GeneArt Strings片段在出厂前都是经过bulk sequence-controlled。
GeneArt Strings DNA片段是客户定制的、未克隆的双链线性DNA片段，它们的长度在100到3000 碱基对（bp）之间，是使用与GeneArt高质量基因合成相同的工艺从合成的寡核苷酸组装而来。GeneArt Strings DNA片段可以用来快速克隆您的目的基因。在5（小于1000bp片段而言）到8（1000至3000 bp片段）个工作日内，可提供200 ng以上的GeneArt Strings DNA片段。GeneArt Strings DNA片段可在线进行设计、序列优化以及订购，是您克隆构建表达质粒的一种经济、明智的替代方法。
The kit and custom services offer different advantages. Please click here (http://www.thermofisher.com/us/en/home/life-science/cloning/gene-synthesis/geneart-gene-synthesis-product-selection-guide.html) to view a selection guide comparing the technologies, including deliverables and production time, to determine what is best for you.
GeneArt Strings fragments are PCR amplicons of assembled oligonucleotides, ready for screening to identify the correct clone. Strings fragments are bulk sequence-controlled as part of the QC process to help ensure that your desired sequence is present in the fragment pool.
To identify a correct clone >90% of the time, we recommend using these screening guidelines:
•Strings fragments up to 1 kb: Sequence 2 to 4 full-length clones
•Strings fragments 1-2 kb: Sequence 3 to 5 full-length clones
•Strings fragments 2-3 kb: Sequence 4 to 8 full-length clones
No. GeneArt Strings DNA fragments are limited to 100 to 3,000 bp. If your sequence is longer, you need to order it as gene synthesis or assemble your gene from two or more GeneArt Strings DNA fragments. In addition, the GeneArt Strings DNA fragments manufacturing process is streamlined to quickly and cost-effectively provide you with your gene product. If your sequence is complex, you may receive a message that it cannot be produced as a Strings DNA fragment due to high complexity. In that case, we recommend modifying your sequence to match the production criteria (given below) or ordering your gene as gene synthesis. In many cases, optimizing your sequence using the portal function enables manufacturing with the GeneArt Strings fragments process. If it is still not able to be produced, you can manually edit the sequence to enable production.
Important production criteria are:
• GC content from 20 to 80% (in peaks, not overall content)
• No long secondary structures or sequence repetitions
• No A/T stretches >25 bp nor G/C stretches >20 bp
Yes, it is possible to directly assemble two GeneArt Strings fragments without pre-cloning. We offer several technologies for seamless assembly, e.g., GeneArt Type IIs Assembly or GeneArt Seamless Cloning and Assembly. Depending on the sequence length and the number of subfragments you want to assemble, the screening effort to find a correct clone can be high. Therefore, we generally recommend pre-cloning the subfragments to limit the screening effort required to find a correct clone after assembly.
No, subcloning and assembly services are not available for GeneArt Strings DNA fragments, as they are designed to offer you the fastest and most affordable way to get access to your genes. If you do not want to clone the gene yourself, we recommend that you order full gene synthesis service.
Yes. We recommend using the GeneArt web portal for ordering, where you can use the free gene editing and optimization functions to adjust the sequence to meet your needs. After editing and optimization, please check again that the final sequences of the fragments are exactly as needed for further processing in your lab (e.g., potential homologous overlaps of the fragments or restriction sites and buffer bases needed for cloning).
No. GeneArt Strings DNA fragments from 150 to 1,000 bp are produced in 5 business days and GeneArt Strings DNA fragments from 1,000 to 3,000 bp are produced in 8 business days. Depending on the nature of the sequence, production time can vary.
GeneArt Strings DNA fragments are shipped dried, ready for resuspension and cloning. Before using, we recommend spinning down the tube contents, adding some water to the bottom of the tube to get the desired concentration, and incubating for at least 1 hour at room temperature (alternatively, incubate at 4°C overnight). Subsequently, we recommend resuspending the fragments carefully and using immediately. For longer storage, resuspended GeneArt Strings DNA fragments should be dispensed into aliquots and frozen at -20°C. Please avoid freeze-thaw cycles.
GeneArt Strings DNA fragments are PCR amplicons of assembled oligonucleotides; an intermediate product of the gene synthesis production process. This process results in a pool of fragments, and cloning and screening need to be carried out to identify the correct clone. To ensure that correct fragments are present in the pool, GeneArt Strings fragments are bulk sequence-controlled before shipment.
GeneArt Strings DNA fragments are custom-made, uncloned, double-stranded linear DNA fragments, available in lengths from 150 to 3,000 base pairs (bp), assembled from synthetic oligonucleotides using the same process developed for GeneArt high-quality gene synthesis. GeneArt Strings fragments can be used to quickly clone your gene of interest. At least 200 ng of GeneArt Strings DNA fragments are produced within 5 business days (up to 1,000 bp) or 8 business days (from 1,000 to 3,000 bp). GeneArt Strings DNA fragments can be designed, sequence-optimized, and ordered online and are a cost-effective and smart alternative to clone your expression plasmid. Please refer to thermofisher.com/strings for further details.