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查看更多产品信息 GeneArt™ Synthetic Gene, 7 to 9 kb, Online Order Rate - FAQs (817009DE)
42 个常见问题解答
TALs是类转录因子的效应蛋白，是天然的转录激活因子。GeneArt Precision TALs允许研究者选择它们想要功能化的精确DNA序列，并且据此构建特定的TAL基因以实现此目的。
请查看以下链接探索我们的合成生物学服务（https://www.thermofisher.com/cn/zh/home/life-science/synthetic-biology/synthetic-biology-services.html），其内容包含：载体构建，cDNA文库构建，蛋白表达服务，定向进化服务，质粒服务，以及细胞系和蛋白服务。
是的，我们将基于您选择的物种对该蛋白进行优化，为您的研究提供经过优化的基因序列。
不需要，您的载体已经被入库并测序，并且在它们被订购/使用一次之后便会出现在您自己的“我的门户载体”列表中。
当预估的完成日期到达时，如果并不是所有的基因均合成完毕，则我们会送出部分订单。未完成的订单会分次按周发送。分批运送不会收取额外费用。
通常，我们会在预估最长完成时间的基因合成完毕后立即投递。在极少数情况下，订单在预估完成时间内没有全部发送。请注意，如果客户将基因合成和亚克隆作为一个过程订购，他们可能最先收到连到Geneart 载体上的目的基因的克隆，然后收到克隆到客户提供的亚克隆载体上的重组质粒。
您可以下载您的FASTA格式的优化序列。它可以作为txt文件/格式读取。FASTA文件将给出对于基因的简单描述，然后给出一个非注释版本的序列。您可以使用免费在线工具将该文件和原始序列进行比对，或者如果您有Vector NTI软件的话，您可以使用它进行比对。
您通常会随订单收到下列内容：载体图谱，比对图谱，测序足迹图。扩展文件可能包括：
1.数据存储证明——保证存储所有生产指标数据
2.材料文件——包括所有使用过的化学物质的信息，包括供货商和货号
3.GLP源文件——包括所有使用过的试剂按批号信息记录的材料颗粒度文件
TSE代表Transmissible Spongiform Encephalopathy（传染性海绵状脑病），意味着无TSE标准的质粒制备所用的培养基是基于大豆的。
对于合成有不同的监管/规则需要遵循，包括使用不同的实验室。在生物安全二级实验室，合成基因需要花费更长时间，因此这类项目费用更高。请注意，无论您如何选择，Thermo Fisher Scientific公司和/或其附属机构都将进行生物安全评估。当您的选择为生物安全1级而我们的评估要求DNA在生物安全2级实验室合成时，您将收到一份根据生物安全2级要求修改的报价单供您审阅。
请提供5-10µg载体DNA用于您的GeneArt用户定制服务。我们建议将DNA溶解在TE缓冲液或水中，或者作为沉淀固体运送。
您将得到两个载体——连有目的基因的基本标准载体，连有目的基因的所选载体。
是的，我们为每个GeneArt合成产品保留一份样本。
除您选择的内切酶位点之外，GeneArt合成过程中添加了标准内切酶位点，用于自动化克隆到GeneArt标准载体上。
没有，并且无法定制。您需要自行亚克隆到您选择的载体。作为定制服务的“Geneart载体”部分，您唯一可选的是抗性标记（需要支付小额标记费）。
在我们的标准载体构建过程中不能随意选择酶切位点进行克隆。在我们的生产过程中，内切酶的引入通常会切去标准载体上的大部分多克隆位点。多克隆位点中包含更多酶切位点的原因是为了允许在某些情况下由于插入序列内部的内切酶位点而需要选择其他酶切位点的情况。
生产时间是基于基因长度和所选择的递送速度。请参见此链接（https://www.thermofisher.com/cn/zh/home/life-science/cloning/gene-synthesis/geneart-gene-synthesis/geneart-standard-and-xxl-genes.html）获知我们的运输状况之间的差别。
GeneArt Strings DNA片段是未克隆的双链DNA片段，长度最长3,000 bp。GeneArt基因合成的产品是已经被克隆在标准GeneArt载体(pMx)内的双链DNA片段。请注意，一段序列可能因为重复序列或高GC含量而不能作为Strings DNA片段进行合成。在这种情况下，我们的科学家建议您通过基因合成订购您的基因。在某些情况下，您可以尝试使用GeneOptimizer算法优化您的序列，有时也是能得以成功优化并作为DNA片段进行订购。
MyGeneObserver显示您的网络订单或非网络订单的制造进程，以及完成时间，以便您能够对您的基因合成项目进行追踪。
Fath et al. (2011) A Standardized Tool to Assess and Enhance Autologous Mammalian Gene Expression.
PLoS ONE 6(3): e17doi:10.1371/journal.pone.0017596
一个项目可以有多个流程，但是可以作为一个项目而订购。以这种方式订购的话则运输/操作费用将仅产生一次。
如果不满足此标准，可以将该基因做为新的基因合成项目订购。
TALs are transcription activator-like effector proteins that are naturally occurring transcriptional activators. GeneArt Precision TALs allow researchers to determine the exact DNA sequence they would like to have their functionality delivered to and have specific TAL genes built to perform the function.
Please use the following link (http://www.thermofisher.com/us/en/home/life-science/synthetic-biology/synthetic-biology-services.html) to explore our synthetic biology services which include: vector construction, cDNA library construction, protein expression services, directed evolution services, plasmid services, and cell line & protein services.
Yes, we will optimize the protein based on the species you choose to provide you with the optimized gene sequence for your studies.
No, your custom vector has been banked and sequenced and is available to you in your individual short list “My Portal Vectors” as soon as they have been ordered/used once.
Partial deliveries can occur when upon reaching the estimated completion date not all order items have been finalized. Partial shipments of incomplete orders are initiated on a weekly basis. Separate shipping of items does not incur an extra cost.
Generally, we ship immediately upon completion of the project item with the longest estimated turnaround time. In rare cases, completely finalized projects are not shipped until the estimated completion date has been reached. Please keep in mind, if you order gene synthesis and subcloning as a process, you will most likely receive the gene synthesis in a Geneart vector before you receive your gene in your subcloned vector.
You can download your optimized sequence in FASTA format. It can be read in a txt file/format. The FASTA file will give a simple description of the gene, and then give a non-annotated version of the sequence. You can align this file with their original sequence using a free online tool, or if you have Vector NTI software, you can use this to align the files.
You typically would receive the following with your order: vector map, alignment maps, sequencing traces. Extended documentation can include:
1. Data storage certificate - guaranteed storage of all production specific data
2. Material documentation - includes all information on all used chemicals including providers and catalog numbers
3. GLP source documentation - includes the granularity of the material documentation to lot number level of all used reagents
TSE stands for Transmissible Spongiform Encephalopathy; ie media is based on soy for TSE-free plasmid preps.
Yes. There are different compliance regulations/rules for synthesis, including the use of different labs. With Biosafety 2, it takes a longer time to synthesize genes, therefore, these projects may be more expensive. Please note, regardless of your selection, Thermo Fisher Scientific Corporation and/or its affiliates will perform a biosafety evaluation. In the case that you select Biosafety Level 1 but our evaluation requires the DNA to be a Biosafety Level 2, you will be sent a modified quotation according to the Biosafety Level 2 requirements for review.
Please provide 5-10µg of vector DNA for your GeneArt custom service. We prefer either dissolved in TE or water, or as a pellet.
You will receive both vectors - your gene in the basic cloning vector, and your gene in the subcloned vector of your choice.
Yes, we keep a sample of every GeneArt-synthesized product.
GeneArt synthesis adds standard restriction sites for automated cloning into GeneArt standard vectors in addition to your selected restriction sites.
No, and this cannot be requested. You would need to subclone into a vector of your choice. The only thing you can request as part of the “Geneart vector” is the antibiotic resistance (for a small markup fee).
We do not offer free choices of restriction sites in our standard production vectors. For our production process, restriction enzymes are added that generally eliminate most of the standard vectors' MCS. The reason why the MCS contains more sites is to allow for fallback scenarios in case certain sites cannot be used due to insert sequence intrinsic restrictions.
Production time is based on gene length and choice of shipment speed. Please see this link (http://www.thermofisher.com/us/en/home/life-science/cloning/gene-synthesis/geneart-gene-synthesis/geneart-standard-and-xxl-genes.html) for the differences between our shipping conditions.
GeneArt Strings DNA Fragments are uncloned double-stranded DNA fragments that are limited to length of 1,000 bp. GeneArt Gene Synthesis products are double-stranded DNA fragments that have been cloned into a standard GeneArt vector (pMx). Please note, a sequence may be too complex to be produced as a Strings DNA Fragment due to repetitions or high GC content. In this case, our scientists will recommend that you order your gene via gene synthesis. You could try optimizing your sequence through the GeneOptimizer algorithm which, in some cases, could optimize the sequence and allow the DNA Fragments to be ordered.
GeneObserver shows the manufacturing status of your web and non-web orders, as well as the completed date so that you can track your gene synthesis project.
Fath et al. (2011) A Standardized Tool to Assess and Enhance Autologous Mammalian Gene Expression. PLoS ONE 6(3): e17doi:10.1371/journal.pone.0017596
A project can have multiple processes, and therefore, can be ordered as single project. In this way shipping/handling are paid only once.
Single variants and variant bundles (more than one variant process) are variants of a master gene. The variant criteria are as follows:
Variants may contain up to four of the following modifications:
- 5'/3' deletion of any length with additional 52 nt of new sequence on each side
- 5'/3' modification of a maximum of 52 nt on each side
- 5'/3' extension of a maximum of 52 nt on each side
- Internal modifications of up to 40 nt (a maximum of 3 of these internal modification blocks are allowed)
- Internal deletions of any length
- Modifications (whether internal or 5´/3´) must be separated by at least 100 bp.
If a gene falls outside of these criteria, it must be created as a separate gene synthesis instead of as a variant.