POROS™ MabCapture™ G Select 树脂
POROS™ MabCapture™ G Select 树脂
Thermo Scientific™

POROS™ MabCapture™ G Select 树脂

POROS™ MabCapture™ G Select 树脂是一款高通量、低价格的蛋白 G 色谱树脂,其利用我们自己的蛋白 G 配体加工而成,以实现高通量纯化性能了解更多信息
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货号容积(公制)
8208450 mL
8208315 mL
货号 82084
价格(CNY)
42,582.00
Each
添加至购物车
容积(公制):
50 mL
请求批量或定制报价
价格(CNY)
42,582.00
Each
添加至购物车
POROS™ MabCapture™ G Select 树脂是一款高通量、低价格的蛋白 G 色谱树脂,其利用我们自己的蛋白 G 配体加工而成,以实现高通量纯化性能。其用于多种抗体亲和纯化方法,是从小鼠、人、牛、山羊和绵羊血清中纯化多克隆 IgG 的理想选择(包括人 IgG3 和小鼠 IgG1 同种型)。POROS MabCapture G Select 树脂在低线性流速下表现出较高的动态结合性能,同时在其他色谱介质产品无法操作的高线性流速下仍能保持出色的结合容量。

利用 POROS MabCapture G 亲和色谱树脂,您可以:
•在线性流速超过 700 cm/hr 时可维持高动态结合容量
• 由于针对抗体结合效率进行了改进,可设计更具灵活性(更短的柱床层高度、更快的流速或两者兼具)的流程
• 与传统琼脂糖或硅基蛋白 G 树脂相比,该树脂可获得特别高的抗体动态结合容量
• 从小鼠、人、牛、山羊和绵羊血清中纯化多克隆 IgG(包括人 IgG3 和小鼠 IgG1 同种型)
• 使用其他色谱分析产品的标准试剂进行消毒和去热原处理
• 显著提高工艺生产率并减少下游瓶颈

蛋白 G 是一种从 G 群链球菌中分离得到的细菌细胞壁蛋白。重组蛋白 G 已经过修饰,消除了与白蛋白的结合能力。蛋白 G 分子包含两个结合结构域。尽管蛋白 A 和蛋白 G 的三级结构非常相似,但其氨基酸组成显著不同,从而具有不同的结合特性。对于大多数哺乳动物 Ig 而言,蛋白 G 比蛋白 A 更具亲和力,尤其是对于某些亚类,包括人 IgG3、小鼠 IgG1 和大鼠 IgG2a。与蛋白 A 不同,蛋白 G 不能结合人 IgM、IgD 和 IgA。

人源化单克隆抗体的发展持续促进生物治疗市场的增长。人们在设法满足单克隆抗体治疗需求的同时,极大地改进了用于生产这些蛋白质的基于细胞的表达系统。因此,这些改进已经使处理瓶颈从上游细胞表达进入下游纯化。POROS MabCapture G Select 色谱树脂旨在突破下游瓶颈,为下游纯化平台提供改进所需的容量、效率和灵活性,同时显著提高工艺的生产率和抗体制备能力。

仅供科研使用。不可用于诊断程序。
规格
适用于(应用)IgG 纯化
产品规格Poros 微球
产品线POROS™
纯化目标IgG 抗体,Ig 抗体
数量50 mL
运输条件湿冰
标准组分蛋白 G
固定相蛋白 G
容积(公制)50 mL
类型G Select 树脂
Unit SizeEach
内容与储存
冷藏储存 (2–8°C)。

常见问题解答 (FAQ)

Are POROS analytical columns compatible with HPLC systems from Waters or Agilent?

All of the pre-packed POROS columns, regardless of column size or resin, have the same 10-32 female fittings on the column. Columns are compatible with Waters and Agilent HPLC systems. You will need 1/16 inch male fittings for the HPLC to attach the column.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

For any POROS analytical column, is the column compatible with an AKTA or Avant purification system?

All of the pre-packed columns, regardless column size or resin, have the same 10-32 female fittings on the column. They are all compatible with the AKTA instrument platform, however due to the column volume of the 2.1 mmD x 30 mmL columns (0.1 mL), these will not perform well in AKTA or Avant purification systems due to the relative size of the hold- up volume in these systems. The smallest column (PEEK or SS) that could feasibly be used in these systems is the 4.6mmD x 50mmL (0.8 mL) format.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What is the physical or mechanical stability of POROS resins?

The physical stability of POROS resins is high. Customers have agitated, with overhead mixing and or bottom gassing for long periods of time (5-10 hours) and have performed hundreds of diaphragm pump passes during stall packs. Keep in mind that agitation with any device that creates grinding and sheer, such as with a magnetic stir bar, is not recommended for any type of resin.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What should I do if a POROS column dries out or air is pumped onto the column?

POROS resins rehydrate quite well. Here is the procedure that we recommend:

1. Flow 5% ethanol over the column at ?350 cm/hour for 3 CVs, followed by water or 0.1 M sodium chloride at 300 cm/hour for 3 CVs.
2. If baseline noise is still present, flow 20% ethanol over the column at 300 cm/hour for 3 CVs, followed by water or 0.1 M sodium chloride at 300 cm/hour for 3 CVs.
3. If you have established column qualification tests and specifications, perform the tests after rewetting.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Can POROS resins be used to purify large biomolecules?

In general POROS resins have behaved better for large biomolecules such as viral particles, fusion proteins, and globular proteins, where shape (not size) matters due to its unique pore structure and large pore structure as compared to traditional resins. As the target molecule size increases, capacities obtained will decrease. The large pore structure of POROS resins, which allows for convective flow (and therefore enhanced diffusion) is especially well suited for the purification of large biomolecules. POROS resins offer the best blend of high capacity (associated with chromatography beads) and improved chromatography efficiency (typically associated with monoliths or membranes). The average pore size for POROS resins is 1,000-3,600 Angstrom (100-360 nm) depending on the base bead/chemistry being utilized. For example, the molecular weight of IgG is 150 kDa and the molecular radius is 55 Angstrom. The molecular weight of IgM is ~900 kDa (pentamer), and the molecular radius is 120 Angstrom. So both of these biomolecules can interact with the pore structure associated with POROS materials.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.