Unraveling mitotic protein networks by 3D multiplexed epitope drug screening.
AuthorsMaier LJ, Kallenberger SM, Jechow K, Waschow M, Eils R, Conrad C
JournalMol Syst Biol
PubMed ID30104419
'Three-dimensional protein localization intricately determines the functional coordination of cellular processes. The complex spatial context of protein landscape has been assessed by multiplexed immunofluorescent staining or mass spectrometry, applied to 2D cell culture with limited physiological relevance or tissue sections. Here, we present 3D SPECS, an automated technology for 3D ... More
Inducible IFN-? Expression for MHC-I Upregulation in Devil Facial Tumor Cells.
AuthorsOng CEB, Lyons AB, Woods GM, Flies AS
JournalFront Immunol
PubMed ID30692995
'The Tasmanian devil facial tumor (DFT) disease has led to an 80% reduction in the wild Tasmanian devil ('
Development by Genetic Immunization of Monovalent Antibodies Against Human Vasoactive Intestinal Peptide Receptor 1 (VPAC1), New Innovative, and Versatile Tools to Study VPAC1 Receptor Function.
AuthorsPeyrassol X, Laeremans T, Lahura V, Debulpaep M, El Hassan H, Steyaert J, Parmentier M, Langer I
JournalFront Endocrinol (Lausanne)
PubMed ID29674997
Multi-membrane spanning proteins, such as G protein-coupled receptors (GPCRs) and ion channels, are extremely difficult to purify as native proteins. Consequently, the generation of antibodies that recognize the native conformation can be challenging. By combining genetic immunization, phage display, and biopanning, we identified a panel of monovalent antibodies (nanobodies) targeting ... More
Cell-free protein synthesis as a novel tool for directed glycoengineering of active erythropoietin.
AuthorsZemella A, Thoring L, Hoffmeister C, Šamalíková M, Ehren P, Wüstenhagen DA, Kubick S
JournalSci Rep
PubMed ID29867209
As one of the most complex post-translational modification, glycosylation is widely involved in cell adhesion, cell proliferation and immune response. Nevertheless glycoproteins with an identical polypeptide backbone mostly differ in their glycosylation patterns. Due to this heterogeneity, the mapping of different glycosylation patterns to their associated function is nearly impossible. ... More
High-Affinity Bent ß
AuthorsFan Z, Kiosses WB, Sun H, Orecchioni M, Ghosheh Y, Zajonc DM, Arnaout MA, Gutierrez E, Groisman A, Ginsberg MH, Ley K