Pierce™ C18 离心吸头和离心柱
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Pierce™ C18 离心吸头和离心柱
Thermo Scientific™

Pierce™ C18 离心吸头和离心柱

Thermo Scientific Pierce C18 离心柱是即用型多孔 C18 反相树脂离心柱，具有出色的结合和回收特性，适用于质谱法和其他方法的肽样品制备。可以使用 Pierce C18了解更多信息
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货号数量产品类型
8485096 吸头离心吸头
8987025柱色谱柱
8987350柱色谱柱
8778296 x 10 µL 吸头移液器吸头
8778496 x 100 µL 吸头移液器吸头
货号 84850
价格（CNY)
2,531.00
飞享价
Ends: 31-Dec-2025
3,124.00
共减 593.00 (19%)
Each
添加至购物车
数量:
96 吸头
产品类型:
离心吸头
请求批量或定制报价
价格（CNY)
2,531.00
飞享价
Ends: 31-Dec-2025
3,124.00
共减 593.00 (19%)
Each
添加至购物车
Thermo Scientific Pierce C18 离心柱是即用型多孔 C18 反相树脂离心柱，具有出色的结合和回收特性，适用于质谱法和其他方法的肽样品制备。

可以使用 Pierce C18 离心柱针对多种应用纯化和浓缩肽样品。每一个离心柱都包含一个多孔 C18 反相树脂，具有优异的结合和回收特性，可适用范围广泛的肽浓度。这种离心柱的形式允许在大约 30 分钟内同时处理多份样品（每份 10 至 150 μL），而无需耗时费力的重复移液或专门的设备。Pierce C18 离心柱可有效地用于处理从 10 ng - 30 μg 蛋白质衍生的肽。灵敏度和检测限取决于下游应用。

C18 离心柱的特点：

•去除干扰污染物—可显著减少信号抑制并改善信噪比和序列覆盖
• 简化优化—处理过程可产生高质量的光谱，并且对于各种反相兼容的污染物都有效
• 稳健—适用于多种上样体积和浓度；无需在应用前减少样品体积
• 便利—易于处理，无需特殊设备即可同时处理多份样品（与需要一次处理一份样品的吸头驱动系统不同）
• 灵敏—特殊的 C18 树脂可实现出色的回收率，即使在样品上样体积较低（pmol 以下）时也是如此

基质辅助激光解析电离 (MALDI) 和电喷雾电离 (ESI) 质谱分析 (MS) 是研究生物化合物的必要条件，因为它们具有较高的灵敏度和质量精度。然而，生物样品中常见的许多缓冲液和化合物对 MALDI-MS 和 ESI-MS 都会产生干扰，从而降低了灵敏度和光谱质量。Pierce C-18 离心柱可在 MS 兼容溶液中去除干扰污染物并释放肽，为辅助转译后修饰的 MS 检查或通过肽图分析鉴定蛋白提供了一种有效工具。
For Research Use Only. Not for use in diagnostic procedures.

基质辅助激光解析电离 (MALDI) 和电喷雾电离 (ESI) 质谱分析 (MS) 是研究生物化合物的必要条件，因为它们具有较高的灵敏度和质量精度。然而，生物样品中常见的许多缓冲液和化合物对 MALDI-MS 和 ESI-MS 都会产生干扰，从而降低了灵敏度和光谱质量。Pierce C-18 离心柱可在兼容 MS 的溶液中去除干扰污染物并释放肽，为辅助转译后修饰的 MS 检查或通过肽图分析鉴定蛋白提供了一种有效工具。

规格
最终产品类型肽
适用于（设备）微量离心机、质谱仪
数量96 吸头
工作流程步骤样品纯化
检测方法质谱分析
形式实心
产品线Pierce™
产品类型离心吸头
原始材料肽, 蛋白酶酶切的蛋白
Unit SizeEach

常见问题解答 (FAQ)

Do you offer the adaptors provided with Pierce C18 Spin Tips (Cat. No. 84850) as a stand-alone product?

Unfortunately, the adaptors provided with Pierce C18 Spin Tips are not sold separately and they are supplied in a smaller quantity because they are meant to be reused.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

Do I need to do C18 clean-up of my mass spectrometry sample before phosphopeptide enrichment?

Yes. C18 clean-up is necessary to remove small molecules that may interfere with phosphopeptide enrichment. Drying after clean-up is also necessary to concentrate the dilute samples and remove C18 organic eluting solvent so samples can be dissolved in the optimized buffers for phosphopeptide binding. Alternative binding buffers are not recommended as phosphopeptide yield and specificity will be affected. For maximal performance, ensure that the clean, dried peptide samples are completely solubilized in binding buffer with agitation for at least 10 min.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

How do I determine if my sample is compatible and ready for LC-MS?

Samples prepared using LC/MS grade reagents are suitable for LC-MS; however, particulates and other small molecules can all interfere with liquid chromatography separation and mass spectrometer source ionization. We recommend visual inspection of samples for particulate matter and using Pierce Peptide Desalting Spin Columns (Cat. Nos. 89851, 89852), Pierce C18 Spin Tips (Cat. No. 84850) or an in-line C18 trap column (https://www.thermofisher.com/order/catalog/product/164564-CMD) to remove non-volatile salts before MS analysis.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

I cleaned up my peptide sample using C18 or desalting columns, but I lost most of my peptides. What went wrong?

Peptides do not bind well to reversed phase resins at neutral pH or in the presence of organic solvents (e.g., acetonitrile). Acidify protein digest samples using formic acid or trifluoroacetic acid (TFA) to pH <3 before desalting. Ensure that samples do not contain organic solvents before and after clean-up by drying them down using a SpeedVac concentrator or equivalent.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

If I already removed small contaminants and detergents at the protein level, do I still need to desalt my peptides prior to mass spectrometry analysis?

Yes. We recommend performing additional cleanup after protein digestion to remove any residual salts or partially digested proteins using Pierce Peptide Desalting Spin Columns (Cat. Nos. 89851, 89852), Pierce C18 Spin Tips (Cat. No. 84850) or an in-line C18 trap column (https://www.thermofisher.com/order/catalog/product/164564-CMD).

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.