Pierce™ 高 pH 值反相肽分离试剂盒
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Pierce™ 高 pH 值反相肽分离试剂盒
Thermo Scientific™

Pierce™ 高 pH 值反相肽分离试剂盒

Thermo Scientific™ Pierce™ 高 pH 值反相肽分离试剂盒通过复杂肽样品的正交肽分离提高了从 LC/MS 分析中进行的蛋白鉴定。•易于使用—以单次使用离心柱形式提供的树脂• 改善蛋白鉴定—了解更多信息
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货号 84868
价格（CNY)
3,082.00
飞享价
Ends: 31-Dec-2025
3,991.00
共减 909.00 (23%)
Each
添加至购物车
请求批量或定制报价
价格（CNY)
3,082.00
飞享价
Ends: 31-Dec-2025
3,991.00
共减 909.00 (23%)
Each
添加至购物车
Thermo Scientific™ Pierce™ 高 pH 值反相肽分离试剂盒通过复杂肽样品的正交肽分离提高了从 LC/MS 分析中进行的蛋白鉴定。

•易于使用—以单次使用离心柱形式提供的树脂
• 改善蛋白鉴定— 与未分离样品相比，使蛋白鉴定增加 ≥50%。
• 可重现—洗脱曲线和组分分离度差异小于 20%
• 优化— 提供最大蛋白鉴定和肽回收率，同时最大程度减少组分重叠的稳健程序
• 兼容—经过多种复杂样品（包括 TMT™-标记肽）验证的试剂

为了实现深度蛋白质组测序，通常需要在 LC/MS 分析之前通过正交维度分离来降低样品复杂性。Pierce 高 pH 值反相肽分离试剂盒利用高 pH 值反相色谱通过疏水性分离多肽，提供出色的正交性至低 pH 值反相 LC-MS 梯度。

该试剂盒设计用于在易于使用的离心柱形式中使用具有专利技术的反相树脂通过高 pH 值分离方案来提高蛋白鉴定。与强阳离子交换 (SCX) 分离相比，高 pH 值反相组分在 LC/MS 分析之前不需要额外的脱盐步骤。

高 pH 值反相肽分离试剂盒包括高 pH 值缓冲液（0.1% 三乙胺）和含有耐 pH 值反相树脂的十二个离心柱。每一个反相分离离心柱可以使用微量离心机对 10 –100 μg 肽样品进行分离。

天然、磷酸化 Tandem Mass TagTM (TMTTM)-标记的以及其他复杂肽混合物样品可以使用该试剂盒进行分离。将单一组分生成的搜索结果相结合，有助于提高蛋白序列覆盖率，并增加相对于未分离样品的已鉴定蛋白的数量。

应用：
•降低样品复杂性，以鉴定靶标
•进行系统生物学研究
•降低样品复杂性，以改进定量研究
For Research Use Only. Not for use in diagnostic procedures.
规格
最终产品类型肽
适用于（设备）小型离心机
数量12 次反应
工作流程步骤分馏
检测方法质谱分析
产品规格试剂盒
产品线Pierce™
产品类型肽分离试剂盒
原始材料肽, 蛋白酶酶切的蛋白
Unit SizeEach

常见问题解答 (FAQ)

Can I purchase the Reversed-Phase Fractionation Spin Columns from the Pierce High pH Reversed-Phase Peptide Fractionation Kit (Cat. No. 84868) separately?

No, the spin columns from this kit cannot be purchased separately. However, if you are only using these columns to remove salts from your peptide samples, you can purchase the Pierce Peptide Desalting Spin Columns (Cat. No. 89851, 89852), as an alternative.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

How much starting material is needed to fractionate my samples using the Pierce High pH Reversed Phase Fractionation Kit (Cat. No. 84868)?

A minimum of 100 µg of protein as starting material from the digestion step is needed for fractionation using eight fractions. Less starting material would be needed if fewer fractions are used. If peptides are being measured, the range of peptide to be loaded on these columns is 10 to 100 µg.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

I want to fractionate a complex mass spectrometry sample, but my sample is different from the one described in the manual for the Pierce High pH Reversed-Phase Peptide Fractionation Kit. Should I use a customized fractionation gradient?

Recommended gradients are available for typtic protein digest samples with or without TMT labeling. Use of other chemical labels, performing selective peptide enrichment, or using a different digestion enzyme other than trypsin may affect the elution profile of the peptides during fractionation. For the best sample peptide coverage, an ideal elution profile would result in relatively equal amounts of peptides in each fraction.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

After running the Pierce TMT11plex Yeast Digest Standard, I observe low signal to noise ratio and poor quantitation accuracy for the Tandem Mass Tag (TMT) reporter ions. What do you recommend?

Here are some recommendations:

- Clean and recalibrate the mass spectrometry instrument using one of our Pierce Calibration Solutions (https://www.thermofisher.com/search/browse/category/us/en/600654).
- Verify settings for liquid chromatography (LC) acquisition methods.
- Fractionate TMT-labeled samples using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (Cat. No. 84868) to reduce sample complexity.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

I have a lower number of peptide/protein identification in my combined TMT-labeled samples compared to the unlabeled sample. What can I do?

We recommend fractionating the sample using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (Cat. No. 84868), to reduce the sample complexity before LC-MS analysis and thus increase the number of quantifiable peptides/proteins for multiplexed samples.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

View all Pierce™ 高 pH 值反相肽分离试剂盒 FAQs