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GeneArt™ Plasmid Premium Prep - FAQs

查看更多产品信息 GeneArt™ Plasmid Premium Prep - FAQs (857021DE)

10 个常见问题解答

在GeneArt快速克隆中，对基因合成的要求是什么？

适合于快速克隆的基因必须小于4 kb并且不复杂（对序列的优化可以降低复杂度）。

GeneArt快速克隆可用的载体有哪些？

目前有下列载体可用：

- pcDNA 3.1(+)载体
- pcDNA3.3-TOPO 载体
- pcDNA3.4-TOPO 载体
- pFastBac1 载体
- pET100/D-TOPO载体
- pET151/D-TOPO载体
- pRSET A载体
- pYes2.1V5-His TOPO载体

使用GeneArt快速克隆服务的话我会收到什么？

您将收到克隆入选定表达载体的合成基因。请注意，不会再有传统GeneArt克隆服务提供的连入pMx载体的基因。

何为GeneArt快速克隆服务？

使用另外一种生产过程，使用预制的表达载体，我们的GeneArt客户定制服务团队可以将您合成的基因直接克隆到所选的Thermo Fisher Scientific表达质粒之内，相比于先将基因克隆到pMx系列载体上的标准工作流程节省了4到5个工作日。请访问此页面（https://www.thermofisher.com/us/en/home/life-science/cloning/gene-synthesis/geneart-plasmid-services/geneart-subcloning-service.html）查看快速克隆和传统克隆之间的区别。

我什么时候应该使用GeneArt 基因合成试剂盒，订购GeneArt Strings DNA片段，或者使用GeneArt 基因合成服务？

试剂盒和定制服务有不同的优点。请点击此处（https://www.thermofisher.com/us/en/home/life-science/cloning/gene-synthesis/geneart-gene-synthesis-product-selection-guide.html）查看一份对技术，递送和生产时间进行比较的选择指南，以决定哪种最适合您。

What are the gene synthesis requirements for Express cloning?

Genes qualifying for Express cloning must be <4 kb and not complex (optimization of your sequence can reduce complexity).

What vectors are available for Express cloning?

The following vectors are currently available:

- pcDNA 3.1(+)vector
- pcDNA3.3-TOPO vector
- pcDNA3.4-TOPO vector
- pFastBac1 vector
- pET100/D-TOPO vector
- pET151/D-TOPO vector
- pRSET A vector
- pYes2.1V5-His TOPO vector

What will I receive if I use your GeneArt Express Cloning Service?

You should receive your synthesized gene in the selected expression vector. Please note, no additional copy in a pMx cloning vector will be provided as with traditional GeneArt Cloning Services.

What is the GeneArt Express Cloning Service?

Using an alternative production procedure with pre-prepared expression vectors, our GeneArt custom service team can clone your synthesized genes directly into selected Thermo Fisher Scientific expression plasmids, saving 4-5 business days compared to the standard workflow with cloning into a pMx series vector first. Please visit this page (http://www.thermofisher.com/us/en/home/life-science/cloning/gene-synthesis/geneart-plasmid-services/geneart-subcloning-service.html) for the differences between Express cloning and Classical cloning.

When should I use a GeneArt Gene Synthesis Kit, order GeneArt Strings DNA Fragments, or use GeneArt Gene Synthesis services?

The kit and custom services offer different advantages. Please click here (http://www.thermofisher.com/us/en/home/life-science/cloning/gene-synthesis/geneart-gene-synthesis-product-selection-guide.html) to view a selection guide comparing the technologies, including deliverables and production time, to determine what is best for you.