Slide-A-Lyzer™ 透析瓶
Slide-A-Lyzer™ 透析瓶
Thermo Scientific™

Slide-A-Lyzer™ 透析瓶

有助于在样品量多达 250 mL 的情况下从蛋白或大分子中去除盐和小污染物。每个 Slide-A-Lyzer 透析瓶都配有浮选盘,当其与瓶颈相连时,可使透析瓶竖直浮起以便简单、安全地处理。
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货号描述
87760透析瓶,2K MWCO
87759透析浮选盘
87761透析瓶,3.5K MWCO
87762透析瓶,10K MWCO
87763透析瓶,20K MWCO
货号 87760
价格(CNY)
4,425.00
Each
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描述:
透析瓶,2K MWCO
请求批量或定制报价
价格(CNY)
4,425.00
Each
添加至购物车
Slide-A-Lyzer 透析瓶(2K、3.5K、10K 或 20K MWCO)有助于在样品量多达 250 mL 的情况下轻松、高效地去除缓冲盐和小污染物。易于使用:通过透析瓶顶部的螺旋盖广口,使用血清移液管或者通过直接倾倒的方式添加和移除样品。无需使用绳结或夹子密封设备。Slide-A-Lyzer 透析瓶是在洁净室条件下加工而成,它使用低蛋白结合率再生纤维素膜,可最大限度地提高样品回收率和纯度。
透析瓶有不同的颜色,对应于不同的透析膜孔径 (MWCO):紫色(20,000道尔顿)、橙色(10,000道尔顿)、粉色(3,500道尔顿)和蓝色(2,000道尔顿)。

Slide-A-Lyzer 透析瓶由两张低结合力再生纤维素透析膜构成,这些膜紧密封贴到惰性塑料瓶身的两侧。膜和瓶材料与大多数实验室缓冲液相容。

Slide-A-Lyzer 透析瓶的特点:
易于使用 — 只需用移液管将样品加入瓶中或直接倒入瓶中,即可开始透析
快速透析 — 透析瓶内室扁平,带两张透析膜,因而具有较高的表面积与体积比,可在8小时到一夜时间内透析 250 mL 样品
高回收率 — 矩形瓶设计通过瓶顶部开口极大程度地提高整个样品量的回收率
多种截留分子量 — 选择最适合样品分子量的膜 MWCO

透析膜规格:
成分:再生纤维素
厚度:0.9–1.2 mil (22.5-30 μm);2K MWCO = 2 mil (50 μm)
使用前需要的水合时间:2分钟
甘油含量:微量(10K MWCO 含量是 21%)
硫含量:0.04–0.17%
重金属含量:微量

使用 Slide-A-Lyzer 透析瓶,可以在8小时到一夜时间内完成典型的低分子量污染物去除、缓冲液置换和脱盐。透析速率取决于多种因素,其中包括样品量、所透析的分子大小和形状、搅拌以及温度。对于每个特定的系统,必须根据经验确定最短透析时间。

For Research Use Only. Not for use in diagnostic procedures.
规格
描述透析瓶,2K MWCO
纤维素膜,透析
产品线Slide-A-Lyzer™
纯化目标缓冲液置换,蛋白质
数量4个烧瓶
产品规格细胞培养瓶 (T75)
截留分子量 (MWCO)2.0 kDa
样品体积(公制)250 mL
Unit SizeEach

常见问题解答 (FAQ)

If the Slide-A-Lyzer Dialysis Cassette is not reusable, why are there four portholes?

It is possible to add reagents to the Slide-A-Lyzer Dialysis Cassette at different times using different portholes, allow a reaction to occur, and then dialyze in the same Slide-A-Lyzer Dialysis Cassette, reducing sample loss through reduced sample handling.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Contaminants are not removed completely from my dialysis experiment using the Slide-A-Lyzer Dialysis Flask. What should I do?

All molecules have different diffusion rates across membranes and may not have acted as other compounds of similar molecular weight. Increase dialysis time and/or number of buffer exchanges performed, or alternatively, use a device containing a higher molecular weight cut-off membrane.

Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.

My filled flask does not float in the dialysis solution. What should I do?

Ensure that the flask capacity was not exceeded and that the flotation ring was attached. In some cases, the density of the solution in the flask may require reduced volumes for proper orientation during dialysis. Refer to the product manual for more specific information.

Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.

Why did my sample volume increase during dialysis?

Most likely it‘s because osmotic pressure from dialyzing from high osmolarity to low osmolarity solutions causes a sample volume increase, as each sample component-including water-will move towards equilibrium concentration on both sides of the membrane.

What is the protein recovery after dialysis when using the 96-well Microdialysis plate?

Typical protein recovery is greater than 90% after dialysis.