If the Slide-A-Lyzer Dialysis Cassette is not reusable, why are there four portholes?
It is possible to add reagents to the Slide-A-Lyzer Dialysis Cassette at different times using different portholes, allow a reaction to occur, and then dialyze in the same Slide-A-Lyzer Dialysis Cassette, reducing sample loss through reduced sample handling.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
Contaminants are not removed completely from my dialysis experiment using the Slide-A-Lyzer Dialysis Flask. What should I do?
All molecules have different diffusion rates across membranes and may not have acted as other compounds of similar molecular weight. Increase dialysis time and/or number of buffer exchanges performed, or alternatively, use a device containing a higher molecular weight cut-off membrane.
Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.
My filled flask does not float in the dialysis solution. What should I do?
Ensure that the flask capacity was not exceeded and that the flotation ring was attached. In some cases, the density of the solution in the flask may require reduced volumes for proper orientation during dialysis. Refer to the product manual for more specific information.
Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.
Why did my sample volume increase during dialysis?
Most likely its because osmotic pressure from dialyzing from high osmolarity to low osmolarity solutions causes a sample volume increase, as each sample component-including water-will move towards equilibrium concentration on both sides of the membrane.
What is the protein recovery after dialysis when using the 96-well Microdialysis plate?
Typical protein recovery is greater than 90% after dialysis.