去污剂去除离心柱与板
去污剂去除离心柱与板
引用和文献 (3)
Thermo Scientific™

去污剂去除离心柱与板

使用去污剂去除树脂可快速去除高浓度去污剂且样品损失极少。建议将 Thermo Scientific HiPPR（高蛋白和肽回收率）树脂用于浓度不超过 100 μg/mL 的蛋白，而 Thermo Scientific Pierce 树脂则适用于高于 100 μg/mL 的浓度。
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货号数量产品线产品类型
8778010 mLPierce树脂
8777625 x 125 µL 色谱柱Pierce离心柱
8777725 x 500 µL 色谱柱Pierce离心柱
877785 x 2 mL 色谱柱Pierce离心柱
877795 x 4 mL 色谱柱Pierce离心柱
883042 个 96 孔板Pierce离心板
883055 mLHiPPR去污剂去除试剂盒
8830624 x 0.1 mL 色谱柱HiPPR离心柱
883072 个 96 孔板HiPPR离心板
货号 87780
价格（CNY)
4,588.00
飞享价
Ends: 31-Dec-2025
5,663.00
共减 1,075.00 (19%)
Each
添加至购物车
数量:
10 mL
产品线:
Pierce
产品类型:
树脂
请求批量或定制报价
价格（CNY)
4,588.00
飞享价
Ends: 31-Dec-2025
5,663.00
共减 1,075.00 (19%)
Each
添加至购物车
用于制备蛋白和肽样品的去污剂和表面活性剂可能干扰 ELISA、等电聚焦和质谱法 (MS) 分析。对于 MS 分析，去除肽样品中的去污剂尤其具有挑战性且至关重要。即使低浓度的去污剂也会污染仪器并干扰柱结合、洗脱和肽电离。Thermo Scientific HiPPR 和 Pierce 去污剂去除树脂适用于从胰蛋白酶酶切物中快速去除常见的去污剂，改善 LC-MS/MS 和 MALDI 分析的结果。
• 经优化 — 去除蛋白或肽样品中 >95% 的去污剂
• 快速 — 样品处理所需时间不到15分钟
• 有效 — 在 ELISA、等电聚焦和质谱分析等下游应用中消除去污剂干扰

Pierce 去污剂去除树脂
Pierce 去污剂去除树脂可高效结合并去除 10 µL 至 1 mL 样品中的高浓度去污剂且样品损失极少。这些微量离心柱预装了不同体积的专有树脂，非常适合处理蛋白或肽浓度高于 100 µg/mL 的样品。

HiPPR 去污剂去除离心柱试剂盒
HiPPR 去污剂去除树脂已针对 1-100 µg/mL 的样品浓度进行优化，特别适合去除常用去污剂，包括浓度为 0.5-1% 的 SDS、Triton™ X-100、NP-40 和 CHAPS。树脂以预装离心柱、96孔过滤离心板和散装树脂（便于定制）形式提供。

可选规格：
• 树脂浆—散装树脂随 0.8 mL 空离心柱提供
• 离心柱—0.1 mL 微量离心柱
• 96孔离心板—预分配的96孔过滤板，与离心和真空歧管系统兼容，用于手动或自动纯化

For Research Use Only. Not for use in diagnostic procedures.
规格
色谱柱类型具有专利技术的树脂、亲和性
描述瓶装
纯化目标去污剂去除
数量10 mL
固定相具有专利技术的配体
足够用于1-2.5 mL 典型的含去污剂样品
形式Liquid
产品线Pierce
产品类型树脂
Unit SizeEach
内容与储存
接收后，储存在 4°C 下。将树脂储存于含 0.15 M NaCl 的 0.05% 叠氮化钠溶液中。

常见问题解答 (FAQ)

I still have detergent present in my sample after protein digestion. Is there anything I can do?

Some detergents can be removed from peptide samples using Pierce Detergent Removal Resin (Cat. No. 87780) or HiPPR Detergent Removal Spin Columns (Cat. No. 88306) in the HIPPR (High Protein and Peptide Recovery) format. However, detergents are more easily removed at the protein level using acetone precipitation, dialysis, or affinity capture using Pierce Detergent Removal Resin and HiPPR Detergent Removal Spin Columns before protein digestion.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

What is the best way to remove detergents from my sample for mass spectrometry analysis?

Detergents are more easily removed at the protein level using acetone precipitation (https://tools.thermofisher.com/content/sfs/brochures/TR0049-Acetone-precipitation.pdf), dialysis, or affinity capture using Pierce Detergent Removal Resin (Cat. No. 87780) or HiPPR Detergent Removal Spin Columns (Cat. No. 88306).

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

引用和文献 (3)

引用和文献
Abstract
Evaluation of the tert-butyl group as a probe for NMR studies of macromolecular complexes
Authors:Voleti R, Bali S, Guerrero J, Smothers J, Springhower C, Acosta GA, Brewer KD, Albericio F, Rizo J.
Journal:J Biomol NMR
PubMed ID:34505210
The development of methyl transverse relaxation optimized spectroscopy has greatly facilitated the study of macromolecular assemblies by solution NMR spectroscopy. However, limited sample solubility and stability has hindered application of this technique to ongoing studies of complexes formed on membranes by the neuronal SNAREs that mediate neurotransmitter release and synaptotagmin-1, ... More
BPP43_05035 is a Brachyspira pilosicoli cell surface adhesin that weakens the integrity of the epithelial barrier during infection.
Authors:Rajan A,Gallego P,Dolan B,Patel P,Dwibedi C,Luis AS,Trillo-Muyo S,Arike L,van der Post S,Simrén M,Pelaseyed T
Journal:Gut microbes
PubMed ID:39349383
The anaerobic spirochete Brachyspira causes intestinal spirochetosis, characterized by the intimate attachment of bacterial cells to the colonic mucosa, potentially leading to symptoms such as diarrhea, abdominal pain, and weight loss. Despite the clinical significance of Brachyspira infections, the mechanism of the interaction between Brachyspira and the colon epithelium is ... More
Effects of GDF6 on active protein synthesis by cells of degenerated intervertebral disc.
Authors:Tam V,Chopra N,Sima S,Chen P,Sharma R,Chan D,Diwan A
Journal:European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society
PubMed ID:39920317
INTRODUCTION: Intervertebral disc degeneration (IVD) is a leading cause of low back pain, a prevalent musculoskeletal condition. IVD degeneration is characterized by the degradation of nucleus pulposus (NP), annulus fibrosus (AF), and cartilage endplates (EP). Growth Differentiation Factor 6 (GDF6), part of the bone morphogenetic protein family, has demonstrated potential ... More