Global subcellular characterization of protein degradation using quantitative proteomics.
AuthorsLarance M, Ahmad Y, Kirkwood KJ, Ly T, Lamond AI
JournalMol Cell Proteomics
PubMed ID23242552
'Protein degradation provides an important regulatory mechanism used to control cell cycle progression and many other cellular pathways. To comprehensively analyze the spatial control of protein degradation in U2OS osteosarcoma cells, we have combined drug treatment and SILAC-based quantitative mass spectrometry with subcellular and protein fractionation. The resulting data set ... More
ApoB-100-containing lipoproteins are major carriers of 3-iodothyronamine in circulation.
AuthorsRoy G, Placzek E, Scanlan TS
JournalJ Biol Chem
PubMed ID22128163
3-Iodothyronamine (T(1)AM) is a biogenic amine derivative of thyroid hormone present in tissue and blood of vertebrates. Approximately 99% of the circulating thyroid hormones are bound to plasma proteins, including three major thyroid hormone-binding proteins, and the question arises as to whether circulating T(1)AM is also bound to serum factors. ... More
Long term potentiation is impaired in membrane glycoprotein CD200-deficient mice: a role for Toll-like receptor activation.
AuthorsCostello DA, Lyons A, Denieffe S, Browne TC, Cox FF, Lynch MA
JournalJ Biol Chem
PubMed ID21835925
The membrane glycoprotein CD200 is expressed on several cell types, including neurons, whereas expression of its receptor, CD200R, is restricted principally to cells of the myeloid lineage, including microglia. The interaction between CD200 and CD200R maintains microglia and macrophages in a quiescent state; therefore, CD200-deficient mice express an inflammatory phenotype ... More