Syn-PER™ Synaptic Protein Extraction Reagent - FAQs

查看更多产品信息 Syn-PER™ Synaptic Protein Extraction Reagent - FAQs (87793)

4 个常见问题解答

Will yield be the same with cultured neurons when using Syn-PER Synaptic Protein Extraction Reagent (Cat. No. 87793), as compared to using fresh tissue?

We can not guarantee that the total synaptic yield from cultured neurons should be comparable to using fresh tissue.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Will yield be the same if I pellet and freeze neurons in culture when using Syn-PER Synaptic Protein Extraction Reagent (Cat. No. 87793), as compared to using fresh tissue?

We have tested using Syn-PER Synaptic Protein Extraction Reagent with frozen (mouse) brain tissue and total synaptic protein yields were comparable to fresh brain tissue.

Please note, frozen tissues are not recommended for synaptosome function tests such as neurotransmitter release and up take assays because synaptosomes from frozen tissues are not viable. This is a difference between frozen and fresh tissue in terms of application.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can I perform RNA extraction after using Syn-PER Synaptic Protein Extraction Reagent (Cat. No. 87793)?

Yes. RNA extraction can be performed after using Syn-PER Synaptic Protein Extraction Reagent (Cat. No. 87793). After the lysis step, you will need to perform two centrifugations (step 4 and 5 in the manual, the first at low speed to discard the tissue or cell debris. The second one, at higher speed, will pellet synaptosomes. The supernatant arising from this step will include the cytosolic fraction (step 6).

If the synaptosomes are intact and have RNA inside, the RNA should be extractable by the kit or method of your choice.

Please note: This extraction procedure should be experimentally tested.

Find additional tips, troubleshooting help, and resources within our RNA Sample Collection, Protection, and Isolation Support Center.

Can you provide the shelf-life for the Syn-PER Synaptic Protein Extraction Reagent?

The Syn-PER Synaptic Protein Extraction Reagent is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended (4 degrees C). Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.