Simultaneous visualization of multiple antigens with tyramide signal amplification using antibodies from the same species.
AuthorsTóth ZE, Mezey E,
JournalJ Histochem Cytochem
PubMed ID17242468
After immunohistochemistry (IHC) began to be used routinely, a number of investigators worked on methods for staining multiple molecules in the same tissue sections or cells. Achieving this goal was not easy, however. One reason for this is that the majority of primary antibodies used in IHC reactions are raised ... More
Sensitive detection of GFP utilizing tyramide signal amplification to overcome gene silencing.
AuthorsToth ZE, Shahar T, Leker R, Szalayova I, Bratincsák A, Key S, Lonyai A, Németh K, Mezey E,
JournalExp Cell Res
PubMed ID17428464
The green fluorescent protein (GFP) is among the most commonly used expression markers in biology. GFP-tagged cells have played a particularly important role in studies of cell lineage. Sensitive detection of GFP is crucially important for such studies to be successful, and problems with detection may account for discrepancies in ... More