Are the Super Bright or Brilliant Violet conjugated antibodies compatible with PrimeFlow RNA Assay Kit (Cat No. 88-18005-210)?
The Brilliant Violet dyes are reported to work with the PrimeFlow RNA Assay kit. For more information on fluorochrome compatibility, please check the following link.
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Do you supply positive control probes as part of the PrimeFlow RNA Assay, Cat. No. 88-18005-204, 88-18005-210?
Positive control probe sets are sold separately. Please refer to Appendix 5 of the PrimeFlow RNA Assay User Manual for recommendations and catalog numbers for recommended positive control genes. However, the appropriate positive control gene for your specific model system should be determined empirically.
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What is the sensitivity for microRNA detection with the PrimeFlow RNA Assay kit with the miRNA Pretreatment Buffrer (Cat. No 88-18006)?
The sensitivity is estimated to be 40 copies per cell with Type 1, Alexa Fluor 647 Probe Sets. The actual sensitivity may vary depending on the specific target and Probe Type.
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Is the microRNA Pretreatment buffer (Cat. No. 88-18006) required for microRNA detection in the PrimeFlow RNA Assay?
The Pretreatment Buffer is not absolutely required for microRNA detection. However, it can enhance detection for most of the microRNA targets tested.
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How does the data look for microRNA staining if we use the standard PrimeFlow RNA Assay protocol (Cat. No. 88-18009 or 88-18001)?
In some cases, there is a decrease in the signal for a given microRNA target, in other cases there is no difference. The extent of the decrease and whether or not it occurs varies depending on the specific microRNA target being analyzed.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
