PrimeFlow™ RNA 检测试剂盒

This PrimeFlow™ RNA Assay is an in situ hybridization assay that combines the power of branched-DNA technology with the single-cell resolution of flow cytometry, enabling the simultaneous detection of up to four RNA targets in combination with immunophenotyping for cell surface and intracellular proteins using fluorochrome-conjugated antibodies. Branched-DNA technology uses pairs of target-specific probes to amplify the detection of a RNA transcript, achieving between 8,000- to 16,000-fold signal amplification from a desired RNA transcript. PrimeFlow RNA Assay can detect mRNA, long non-coding RNA, viral RNA, and telomere DNA. The detection of microRNA is also possible and is optimal when using the PrimeFlow™ microRNA Pretreatment Buffer (cat. 88-18006) and protocol. PrimeFlow RNA Assay is compatible with human and mouse cells. Other mammalian and non-mammalian cells may be compatible

This PrimeFlow RNA Assay contains all the reagents needed to conduct the assay. Positive control probes and target probes for genes of interest are sold separately.

Reported Application
RNA Flow Cytometry

PrimeFlow™ RNA 检测试剂盒是一种原位杂交测定试剂盒，其将分支 DNA 技术的强大功能与流式细胞分析的单细胞分辨率相结合，可同时检测多达四种 RNA 靶标，并兼容使用荧光染料结合抗体检测细胞表面和细胞内蛋白的免疫表型。分支 DNA 技术采用成对靶标特异性探针扩增 RNA 转录本检测，从而实现目标 RNA 转录物 8,000 到 16,000 倍的信号放大。PrimeFlow RNA 检测试剂盒可以检测 mRNA、非编码长链 RNA、病毒 RNA 和端粒 DNA。也可以用来检测 microRNA，使用 PrimeFlow™ microRNA 预处理缓冲液（货号88-18006）和方案时较佳。PrimeFlow RNA 检测试剂盒适用于检测人和小鼠细胞。也可能兼容其他哺乳动物和非哺乳动物细胞

PrimeFlow RNA 试剂盒含有测定所需的所有试剂。阳性对照探针和目标基因的靶标探针单独出售。

已报告应用
RNA 流式细胞分析