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Invitrogen
Rat IgM Uncoated ELISA Kit
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产品规格
检测灵敏度
0.3125 ng/mL
测定范围
0.3125-20 ng/mL
检测样本类型/体积
{Plasma=10 µL, Serum=10 µL, Supernatant=10 µL}
手工操作时间
1 hr 45 min
总时间
24 hr 30 min
均相（无需洗涤）
No
检测仪器
Absorbance Microplate Reader
产品规格
10 x 96 Tests
产品成分
Capture Antibody: Pre-titrated, purified antibody
Detection Antibody: Pre-titrated, HRP-conjugated antibody
Standard: Recombinant protein for generating standard curve and calibrating samples
10X Coating Buffer: Buffer for plating the Capture Antibody
5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme
Substrate: 1X TMB Solution
Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards
96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
Detection Antibody: Pre-titrated, HRP-conjugated antibody
Standard: Recombinant protein for generating standard curve and calibrating samples
10X Coating Buffer: Buffer for plating the Capture Antibody
5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme
Substrate: 1X TMB Solution
Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards
96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
运输条件
Wet ice
储存
2-8°C
蛋白名称
Rat IgM
Target site-specificity
检测试剂盒格式
Uncoated ELISA Kit
检测抗体结合物
HRP
标签或染料
HRP
关于本试剂盒
The Rat IgM Uncoated ELISA Kit contains pre-matched antibody pairs and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of rat IgM. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.
原理方法
ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
靶标信息
Rat IgM is an immunoglobulin M isotype antibody produced by B cells in rats. It plays a crucial role in the immune system by recognizing and neutralizing antigens, such as bacteria and viruses. Rat IgM has been extensively studied and is widely used in research applications, including immunology, microbiology, and biotechnology. It is commonly used as a control in experiments, as well as a diagnostic tool for detecting infectious diseases and autoimmune disorders. Researchers have developed various techniques for producing and purifying Rat IgM, including hybridoma technology, protein A/G chromatography, and affinity chromatography. Rat IgM is a valuable tool in the field of immunology, and its use has contributed to a better understanding of the immune system and the development of new treatments for various diseases.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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