Selective binding, magnetic separation and purification of histidine-tagged protein using biopolymer magnetic core-shell nanoparticles.
AuthorsZhou Y,Yan D,Yuan S,Chen Y,Fletcher EE,Shi H,Han B
JournalProtein expression and purification
PubMed ID29154996
In previous studies, we synthesized the magnetic core-shell structured Fe(3)O(4)/PMG/IDA-Ni(2+) nanoparticles. The Ni(2+) on the surface of nanoparticles provides abundant docking sites for histidine, and the composite nanoparticles showed potential applications in the separation and purification of histidine-tagged (His-tagged) proteins. Meanwhile, the presence of the superparamagnetic core (Fe(3)O(4)) in the ... More
Diffusion and capture permits dynamic coupling between treadmilling FtsZ filaments and cell division proteins.
AuthorsBaranova N,Radler P,Hernández-Rocamora VM,Alfonso C,López-Pelegrín M,Rivas G,Vollmer W,Loose M
JournalNature microbiology
PubMed ID31959972
Most bacteria accomplish cell division with the help of a dynamic protein complex called the divisome, which spans the cell envelope in the plane of division. Assembly and activation of this machinery are coordinated by the tubulin-related GTPase FtsZ, which was found to form treadmilling filaments on supported bilayers in ... More