Plate Seal for Pierce™ 96-well Microdialysis Plates - FAQs

Q: Why did my sample volume increase during dialysis?

Most likely its because osmotic pressure from dialyzing from high osmolarity to low osmolarity solutions causes a sample volume increase, as each sample component-including water-will move towards equilibrium concentration on both sides of the membrane.

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5 个常见问题解答

Why did my sample volume increase during dialysis?

Most likely it‘s because osmotic pressure from dialyzing from high osmolarity to low osmolarity solutions causes a sample volume increase, as each sample component-including water-will move towards equilibrium concentration on both sides of the membrane.

What is the protein recovery after dialysis when using the 96-well Microdialysis plate?

Typical protein recovery is greater than 90% after dialysis.

Do I need to use all 96 wells in the Pierce 96-well microdalysis plate at one time?

The plate comprises detachable strips so that you can pull out the exact number of wells needed for an experiment (scalable from 1-96 samples).

What is the difference between your dialysis devices (plates, device, cassettes, flasks)?

Please view our selection table to choose the right dialysis device for your experiment - https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-purification-isolation/protein-dialysis-desalting-concentration/dialysis-products.html.

What is protein dialysis?

Dialysis is the separation of small and large molecules in a solution by selective diffusion through a semi-permeable membrane. It is generally used for larger sample volumes, and can take hours to overnight for complete dialysis.