Maternal pre-conceptional glyphosate exposure impacts the offspring hepatic and ovarian proteome.
AuthorsNovbatova G,Timme K,Severin A,Sayadi M,Keating AF
JournalToxicological sciences : an official journal of the Society of Toxicology
PubMed ID37208198
Glyphosate (GLY) is an herbicide used for rural and urban weed control. Urinary GLY in women is associated with shortened gestational length yet effects of GLY on offspring due to maternal exposure are unclear. This study tested the hypothesis that maternal chronic pre-conceptional GLY exposure would cause phenotypic and molecular ... More
Soybean seed proteomics: Methods for the isolation, detection, and identification of low abundance proteins.
AuthorsKim W,Nott J,Kim S,Krishnan HB
JournalMethods in enzymology
PubMed ID36280356
The salt-soluble globulins, glycinins (11S globulin), and β-conglycinins (7S globulin), are the most abundant seed proteins of soybean seeds. Together, these two groups of proteins account for 60-70% of total soybean seed proteins. Proteomic assessment of the less abundant soybean seed proteins using general isolation protocols is challenging due to ... More
Hybrid Quadrupole Mass Filter-Radial Ejection Linear Ion Trap and Intelligent Data Acquisition Enable Highly Multiplex Targeted Proteomics.
Targeted mass spectrometry (MS) methods are powerful tools for the selective and sensitive analysis of peptides identified in global discovery experiments. Selected reaction monitoring (SRM) is the most widely accepted clinical MS method due to its reliability and performance. However, SRM and parallel reaction monitoring (PRM) are limited in throughput ... More