Untargeted metabolomics to understand the basis of phenotypic differences in amphotericin B-resistant Leishmania parasites.
AuthorsPountain AW,Barrett MP
JournalWellcome open research
PubMed ID32133420
Background: Protozoan Leishmania parasites are responsible for a range of clinical infections that represent a substantial challenge for global health. Amphotericin B (AmB) is increasingly used to treat Leishmania infection, so understanding the potential for resistance to this drug is an important priority. Previously we described four independently-derived AmB-resistant L. ... More
Identification of Phenolic Compounds from Hancornia speciosa (Apocynaceae) Leaves by UHPLC Orbitrap-HRMS.
Apocynaceae is a botanical family distributed mainly in tropical and subtropical regions of the world. In Brazil, they comprise about 90 genera and 850 species, inhabiting various types of vegetation. Within this large botanical family, the genus Hancornia is considered monotypic, with its only species Hancornia speciosa Gomes. Antihypertensive, antidiabetic, ... More
Protocol for identifying metabolite biomarkers in patient uterine fluid for early ovarian cancer detection.
AuthorsJiang Y,Yue W,Bi M,Guo Y,Gu X,Li M
JournalSTAR protocols
PubMed ID38489270
High mortality of ovarian cancer (OC) is primarily attributed to the lack of effective early detection methods. Uterine fluid, pooling molecules from neighboring ovaries, presents an organ-specific advantage over conventional blood samples. Here, we present a protocol for identifying metabolite biomarkers in uterine fluid for early OC detection. We describe ... More
Tulasporins A-D, 19-Residue Peptaibols from the Mycoparasitic Fungus Sepedonium tulasneanum
AuthorsOtto A, Laub A, Haid M, Porzel A, Schmidt J, Wessjohann L, Arnold N
JournalNat Prod Commun
PubMed ID30508342
Four new 19-residue peptaibols, named tulasporins A-D (1-4), were isolated from the semi-solid cultures of Sepedonium tulasneanum. Their structures were elucidated on the basis of extensive ESI-HRMS(n) fragmentation studies as well as (1)H NMR spectroscopic analyses. Interestingly, the structures of tulasporins A-D (1-4) resemble those of chrysospermins isolated earlier from ... More
Mapping small metabolite changes after traumatic brain injury using AP-MALDI MSI.
Traumatic brain injury (TBI) is an alteration of brain function caused by a sudden transmission of an external force to the head. The biomechanical impact induces acute and chronic metabolic changes that highly contribute to injury evolution and outcome. TBI heterogeneity calls for approaches allowing the mapping of regional molecular ... More