Tracking proteasome degradation: A cross-organ analysis via intact degradomics mass spectrometry
AuthorsZittlau KI, Zachor-Movshovitz D, Leushkin Y, Schimmel Brener R, Morgenstern D, Ben-Nissan G, Sharon M.
JournalProc Natl Acad Sci U S A
PubMed ID39964708
The proteasome is a multisubunit degradation machinery that is essential for maintaining protein homeostasis by breaking down unnecessary or damaged proteins into peptides. While most of these peptides are further processed into amino acids, a subset evades complete degradation and plays key roles in biological processes such as antigen presentation, ... More
UHPLC-MS/MS-Based Identity Confirmation of Amino Acids Involved in Response to and Side Effects from Antiseizure Medications.
Real-time breath analysis using secondary electrospray ionization coupled with high-resolution mass spectrometry is a fast and noninvasive method to access the metabolic state of a person. However, it lacks the ability to unequivocally assign mass spectral features to compounds due to the absence of chromatographic separation. This can be overcomed ... More
Development and Validation of Ion Chromatography-Tandem Mass Spectrometry-Based Method for the Multiresidue Determination of Polar Ionic Pesticides in Food.
AuthorsAdams S,Guest J,Dickinson M,Fussell RJ,Beck J,Schoutsen F
JournalJournal of agricultural and food chemistry
PubMed ID28388055
An extraction method using acidified methanol based on the quick polar pesticide (QuPPe) method using suppressed ion chromatography coupled to mass spectrometry was developed and validated for the direct analysis of polar pesticides, without the need for derivatization or ion pairing, in cereals and grapes. The method was robust, and ... More