用于 SILAC 的粉状 RPMI 培养基

Thermo Scientific Powdered RPMI Media for SILAC is optimized for use with stable isotope labeling with amino acids in cell culture (SILAC) to analyze protein expression by mass spectrometry (MS).

Features of Powdered RPMI Media for SILAC:

• Flexible—powdered media deficient in L-leucine, L-lysine and L-arginine, allowing for more complete proteome coverage through multiple isotopic amino acid labeling
• High quality—media are sterile, endotoxin-free and cell culture-compatible

Stable isotope labeling with amino acids in cell culture (SILAC) is a powerful method to identify and quantify relative differential changes in complex protein samples. This approach entails the in vivo metabolic incorporation of 'heavy' ¹³C- or ¹⁵N-labeled amino acids into proteins followed by mass spectrometry (MS) for the accelerated and comprehensive identification, characterization and quantitation of proteins.

This SILAC media is used together with heavy and light amino acids for the differential isotopic labeling of cells for SILAC analysis. SILAC media supplemented with heavy amino acids are capable of nearly 100% label incorporation into proteins of living cells.