PVDF Transfer Membranes, 0.2 μm, 1 Roll
PVDF Transfer Membranes, 0.2 μm, 1 Roll
Thermo Scientific™

PVDF Transfer Membranes, 0.2 μm, 1 Roll

Pierce PVDF Transfer Membranes are made of high-quality polyvinylidene difluoride and provide high binding capacity for proteins and nucleic acids for western, Southern, and northern blotting methods.
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货号数量
885201 Roll
货号 88520
价格（CNY)
8,286.00
Each
添加至购物车
数量:
1 Roll
请求批量或定制报价
价格（CNY)
8,286.00
Each
添加至购物车
Pierce PVDF Transfer Membranes are made of high-quality polyvinylidene difluoride and provide high binding capacity for proteins and nucleic acids for western, Southern, and northern blotting methods. The 0.2-μm pore size is ideal for protein analysis of small amounts of proteins (as little as 10 pmoles), amino acid analysis, and transfer of small molecular weight proteins and peptides. For best results, preactivate the membrane with 100% alcohol.

Features include:
• Re-probe characteristics—high mechanical strength makes PVDF an excellent membrane for stripping and re-probing
• Durability—compatible with most organic solvents, acids, and mild bases
• Compatible with commonly used transfer conditions and detection methods such as staining, chemiluminescence, and radiolabeling. Choose Low-Fluorescence PVDF Transfer Membrane for fluorescent western blotting
• High sensitivity—exceptional binding capacity makes PVDF the ultimate choice for low abundance proteins

For Research Use Only. Not for use in diagnostic procedures.
规格
结合功能Goat IgG: 448 μg/cm2, BSA: 340 μg/cm2, Insulin: 262 μg/cm2
产品类型PVDF Transfer Membrane
数量1 Roll
运输条件Room Temperature
尺寸 (长 x 宽)3.75 m x 26.5 cm
产品规格Roll
长度（公制）3.75 m
材质PVDF
孔径0.2 μm
足够用于≥100 Mini-gel Blots (When cut to 8.8 x 10 cm); ≥80 Midi-gel Blots (When cut to 8.5 x 13.25 cm)
宽度（公制）26.5 cm
Unit SizeEach
内容与储存
Store membranes flat at ambient temperature and away from chemical vapors. Some solvent vapors may partially dissolve the membranes, which will disrupt the pore structure.

常见问题解答 (FAQ)

我将蛋白质转印到PVDF膜上后，转印效率很低。你们有何建议？

以下是可能原因和解决方案：

- 转印前对膜的处理不当：应确保使用甲醇或乙醇等极性有机溶剂润湿膜。
- 凝胶与膜的接触不良：应确保用转膜缓冲液浸透滤纸和海绵垫，小心除去组装膜三明治时的所有气泡。凝胶/膜三明治必须牢固装在两部分印迹模块之间。可尝试多加一个海绵垫或将失去弹性的海绵垫换成新的。
- 过度挤压凝胶：过度挤压的一个明显表现是凝胶过于扁平。在三明治被过度挤压的情况下，应适当移除海绵垫，降低对凝胶和膜施加的过多压力然后关闭转印仪。

你们的PVDF和硝化纤维素膜能否用于Li-COR仪器？

可以，我们的PVDF和硝化纤维素膜均可用于Li-COR仪器。

我正在计划做斑点印迹实验，但我的样品中含有乙腈。你们的PVDF和硝化纤维素膜是否耐受乙腈？

我们的PVDF膜可以耐受乙腈，但硝化纤维素膜不能耐受。

我该如何降低PVDF免疫印迹膜的背景？

PVDF膜需要更为严格的封闭步骤。这可通过将封闭剂的浓度增加2-5倍、延长封闭时间并在37℃封闭来实现。封闭剂与无蛋白质结合的位置结合，可防止背景染色，并封闭与膜结合的蛋白质，以减少其与一抗发生非特异性相互作。封闭剂可使用脱脂奶粉、BSA和酪蛋白。

How can I store, strip, and reuse my western blot?

For nitrocellulose or PVDF membrane following Western blot detection using a chemiluminescent or fluorescent substrate system: Following transfer, air dry the membrane and place in an envelope, preferably on top of a supported surface to keep the membrane flat. The blot can be stored indefinitely at -80 degrees C. When ready to reprobe, prewet the PVDF blot with alcohol for a few seconds, followed by a few rinses with pure water to reduce the alcohol concentration. Then proceed as normal with blocking step.

FOR STRIPPING/REPROBING OF MEMBRANES: Harsh protocol (see NOTE below for modifications)

1) Submerge the membrane in stripping buffer (100 mM BME, 2% SDS, 62.5 mM Tris-HCl, pH 6.7) and incubate at 50 degrees C for 30 min with occasional agitation. If more stringent conditions necessary, incubate at 70 degrees C.

2) Wash 2 x 10 min in TBS-T/PBS-T at room temperature.

3) Block the membrane by immersing in 5% blocking reagent TBS-T or PBS-T for 1 hr at room temperature.

4) Immunodetection

NOTE: Often you don't need such harsh conditions to remove antibodies from their proteins. The stringency of one or several of the variables can be decreased: lower the temperature, decrease the time, less BME, less SDS, etc. An especially mild but still often effective stripping protocol is lower pH incubation. Example: pH 2.0 Tris 50-100 mM, 30-60 min incubation (you may do two incubations if you wish). Then rinse and block as usual. If you do not wish to re-use the membrane immediately after stripping, you can store the membrane in plastic wrap (wet, you do not want it to dry out). Another simple, mild stripping buffer is 0.1 M glycine•HCl (pH 2.5-3.0), incubation 30 min to 2 hrs room temperature or 37 degrees C, depending on the antibody.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

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