Acute lung injury (ALI)/acute respiratory distress syndrome (ARDS) is a life-threatening condition with a high mortality rate of ≈40%. Thyroid hormones (THs) play crucial roles in maintaining homeostasis of the cellular microenvironment under stress. The previous studies confirmed that the clinical-stage TH analog GC-1 significantly alleviates pulmonary fibrosis by improving ... More
Diabetes-induced cognitive dysfunction (DCD) is a neurological disorder associated with diabetes, characterized by cognitive impairment driven by neuronal injury from chronic high glucose (HG) exposure. This study aims to elucidate the role and mechanisms of the X-linked inhibitor of apoptosis protein (XIAP)/thioredoxin-interacting protein (TXNIP) in hippocampal neuron cell death and ... More
Interplay Between Phosphorylation and O-GlcNAcylation of Sarcomeric Proteins in Ischemic Heart Failure.
AuthorsMercier T,Bouvet M,Dubois-Deruy E,Dechaumes A,Beseme O,Richard V,Mulder P,Pinet F
JournalFrontiers in endocrinology
PubMed ID30344511
Post-translational modifications (PTMs) of sarcomeric proteins could participate to left ventricular (LV) remodeling and contractile dysfunction leading in advanced heart failure (HF) with altered ejection fraction. Using an experimental rat model of HF (ligation of left coronary artery) and phosphoproteomic analysis, we identified an increase of desmin phosphorylation and a ... More
Extracellular matrix stiffness regulates degradation of MST2 via SCF (βTrCP).
JournalBiochimica et biophysica acta. General subjects
PubMed ID36044955
The Hippo pathway plays central roles in relaying mechanical signals during development and tumorigenesis, but how the proteostasis of the Hippo kinase MST2 is regulated remains unknown. Here, we found that chemical inhibition of proteasomal proteolysis resulted in increased levels of MST2 in human breast epithelial cells. MST2 binds SCF(βTrCP) ... More
Interaction of cochlin and mechanosensitive channel TREK-1 in trabecular meshwork cells influences the regulation of intraocular pressure.
AuthorsCarreon TA,Castellanos A,Gasull X,Bhattacharya SK
JournalScientific reports
PubMed ID28352076
In the eye, intraocular pressure (IOP) is tightly regulated and its persistent increase leads to ocular hypertension and glaucoma. We have previously shown that trabecular meshwork (TM) cells might detect aqueous humor fluid shear stress via interaction of the extracellular matrix (ECM) protein cochlin with the cell surface bound and ... More
Addendum: MCC950 closes the active conformation of NLRP3 to an inactive state.
AuthorsTapia-Abellán A,Angosto-Bazarra D,Martínez-Banaclocha H,de Torre-Minguela C,Cerón-Carrasco JP,Pérez-Sánchez H,Arostegui JI,Pelegrin P
JournalNature chemical biology
PubMed ID33495649
An Addendum to this paper has been published: https://doi.org/10.1038/s41589-021-00741-6.
Prokaryotic expression of chimeric GFP-hFc protein as a potential immune-based tool.
AuthorsNguyen TT,Vo-Nguyen HV,Tran-Van H
JournalMolecular biology research communications
PubMed ID34476263
GFP is an old-yet-powerful protein marker, which has been widely used in molecular biotechnology due to its capacity of exhibiting bright green fluorescence when exposed to ultraviolet light. The hFc region of IgG antibodies is a specific binding ligand of expressed receptors on immune cells with well-known cellular-associated functions like ... More
The <em>N<sup>6</sup></em>-methyladenosine RNA-binding protein YTHDF1 modulates the translation of <em>TRAF6</em> to mediate the intestinal immune response
AuthorsZong X, Xiao X, Shen B, Jiang Q, Wang H, Lu Z, Wang F, Jin M, Min J, Wang F, Wang Y
JournalNucleic Acids Res
PubMed ID33999206
AKT3-mediated IWS1 phosphorylation promotes the proliferation of EGFR-mutant lung adenocarcinomas through cell cycle-regulated <em>U2AF2</em> RNA splicing
AuthorsLaliotis GI, Chavdoula E, Paraskevopoulou MD, Kaba A, La Ferlita A, Singh S, Anastas V, Nair II KA, Orlacchio A, Taraslia V, Vlachos I, Capece M, Hatzigeorgiou A, Palmieri D, Tsatsanis C, Alaimo S, Sehgal L, Carbone DP, Coppola V, Tsichlis PN
JournalNat Commun
PubMed ID
Combining mass spectrometry and genetic labeling in mice to report TRP channel expression
AuthorsWartenberg P, Lux F, Busch K, Fecher-Trost C, Wyatt A, Flockerzi V, Krasteva-Christ G, Boehm U, Weissgerber P
JournalMethodsX
PubMed ID
Response of human macrophages to gamma radiation is mediated via expression of endogenous retroviruses
AuthorsMikhalkevich N, O’Carroll IP, Tkavc R, Lund K, Sukumar G, Dalgard C, Johnson KR, Li W, Wang T, Nath A, Lordanskiy S