Proximity Labeling of the Chlamydia trachomatis Inclusion Membrane.
AuthorsOlson MG,Jorgenson LM,Widner RE,Rucks EA
JournalMethods in molecular biology (Clifton, N.J.)
PubMed ID31385281
In the study of intracellular bacteria that reside within a membrane-bound vacuole, there are many questions related to how prokaryotic or eukaryotic transmembrane or membrane-associated proteins are organized and function within the membranes of these pathogen-containing vacuoles. Yet this host-pathogen interaction interface has proven difficult to experimentally resolve. For example, ... More
The rapid proximity labeling system PhastID identifies ATP6AP1 as an unconventional GEF for Rheb.
Rheb is a small G protein that functions as the direct activator of the mechanistic target of rapamycin complex 1 (mTORC1) to coordinate signaling cascades in response to nutrients and growth factors. Despite extensive studies, the guanine nucleotide exchange factor (GEF) that directly activates Rheb remains unclear, at least in ... More
Proximity labeling reveals dynamic changes in the SQSTM1 protein network.
AuthorsRondón-Ortiz AN,Zhang L,Ash PEA,Basu A,Puri S,van der Spek SJF,Wang Z,Dorrian L,Emili A,Wolozin B
JournalThe Journal of biological chemistry
PubMed ID39098523
Sequestosome1 (SQSTM1) is an autophagy receptor that mediates the degradation of intracellular cargo, including protein aggregates, through multiple protein interactions. These interactions form the SQSTM1 protein network, and these interactions are mediated by SQSTM1 functional interaction domains, which include LIR, PB1, UBA, and KIR. Technological advances in cell biology continue ... More
Isolation of secreted proteins from Drosophila ovaries and embryos through in vivo BirA-mediated biotinylation
AuthorsStevens LM, Zhang Y, Volnov Y, Chen G, Stein DS
JournalPLoS ONE
PubMed ID
Siglec-E retards atherosclerosis by inhibiting CD36-mediate foam cell formation
The role of the PZP domain of AF10 in acute leukemia driven by AF10 translocations
AuthorsKlein BJ, Deshpande A, Cox KL, Xuan F, Zandian M, Barbosa K, Khanal S, Tong Q, Zhang Y, Zhang P, Sinha A, Bohlander SK, Shi X, Wen H, Poirier MG, Deshpande A, Kutateladze TG