JournalBiochemical and biophysical research communications
PubMed ID31813546
Hypoxia-inducible factor-1α (HIF-1α) induces cancer metastasis. We previously demonstrated that HIF-1α-induced membrane-type 4 matrix metalloproteinase (MT4-MMP) is involved in hypoxia-mediated metastasis in head and neck squamous cell carcinoma (HNSCC). However, the functions and detailed mechanisms of MT4-MMP in cancer metastasis are not well understood. In this study, we investigated whether ... More
A bead-based GPCR phosphorylation immunoassay for high-throughput ligand profiling and GRK inhibitor screening.
Analysis of agonist-driven phosphorylation of G protein-coupled receptors (GPCRs) can provide valuable insights into the receptor activation state and ligand pharmacology. However, to date, assessment of GPCR phosphorylation using high-throughput applications has been challenging. We have developed and validated a bead-based immunoassay for the quantitative assessment of agonist-induced GPCR phosphorylation ... More
Labeling and isolating cell specific neuronal mitochondria and their functional analysis in mice post stroke.
AuthorsLi Y,Zhang ZG,Chopp M,Liu Z,Golembieski W,Landschoot-Ward J,Zhang Y,Liu XS,Xin H
JournalExperimental neurology
PubMed ID39719208
Dendritic and axonal plasticity, which mediates neurobiological recovery after a stroke, critically depends on the mitochondrial function of neurons. To investigate, in vivo, neuronal mitochondrial function at the stroke recovery stage, we employed Mito-tag mice combined with cerebral cortical infection of AAV9 produced from plasmids carrying Cre-recombinase controlled by two ... More
Bi-allelic LAMP3 variants in childhood interstitial lung disease: a surfactant-related disease.
BACKGROUND: LAMP3 encodes a lysosomal membrane protein associated with lamellar bodies and has recently been proposed as a candidate gene for childhood interstitial lung diseases (chILD). Here, we identified two LAMP3 variants in a proband with chILD and performed functional validation of these variants as well as the previously reported ... More
A novel interaction between RNA m(6)A methyltransferase METTL3 and RREB1.
AuthorsXu J,Sivakumar C,Ryan CW,Rao RC
JournalBiochemical and biophysical research communications
PubMed ID39278095
Regulation of gene expression is achieved through the modulation of regulatory inputs both pre- and post-transcriptionally. Methyltransferase-like 3 (METTL3) is a key player in pre-mRNA processing, actively catalyzing N6-methyladenosine (m6A). Among the most enriched mRNA targets of METTL3 is the Ras Responsive Element Binding Protein 1 (RREB1), a transcription factor ... More
Abrogation of USP7 is an alternative strategy to downregulate PD-L1 and sensitize gastric cancer cells to T cells killing.
Targeting immune checkpoints such as programmed cell death protein 1 (PD-1) and programmed death ligand-1 (PD-L1) have been approved for treating melanoma, gastric cancer (GC) and bladder cancer with clinical benefit. Nevertheless, many patients failed to respond to anti-PD-1/PD-L1 treatment, so it is necessary to seek an alternative strategy for ... More
Telomere Length Determines TERRA and R-Loop Regulation through the Cell Cycle
AuthorsMarco Graf, Diego Bonetti, Arianna Lockhart, Kamar Serhal, Vanessa Kellner, André Maicher, Pascale Jolivet, Maria Teresa Teixeira, Brian Luke
JournalCell
PubMed ID
SDE2 integrates into the TIMELESS-TIPIN complex to protect stalled replication forks
AuthorsRageul J, park JJ, Zeng PP, Lee E-A, Yang J, Hwant S, Lo N, Weinheimer, AS, Scharer OD, Yeo J-E, Kim H