Pierce™ 抗 HA 磁珠
Invitrogen17万+抗体限时买二赠一,靶点广,灵活用!
Invitrogen17万+抗体限时买二赠一,靶点广,灵活用!
Pierce™ 抗 HA 磁珠
Thermo Scientific™

Pierce™ 抗 HA 磁珠

Thermo Scientific Pierce 抗 HA 磁珠是用于借助手动或自动磁性分离器对细菌或哺乳动物细胞或体外系统中表达的重组 HA 标签蛋白进行免疫沉淀的亲和微粒。抗 HA了解更多信息
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货号数量
888375 mL
888361 mL
货号 88837
价格(CNY)
11,674.00
Each
添加至购物车
数量:
5 mL
请求批量或定制报价
价格(CNY)
11,674.00
Each
添加至购物车
Thermo Scientific Pierce 抗 HA 磁珠是用于借助手动或自动磁性分离器对细菌或哺乳动物细胞或体外系统中表达的重组 HA 标签蛋白进行免疫沉淀的亲和微粒。

抗 HA 磁珠的特点:

特异性—高度特异性抗 HA 单克隆抗体(克隆 2-2.2.14)可实现高得率和高纯度的免疫沉淀
便利快速—产品说明提供了一个易于遵循的优化方案,可在大约一小时内完成免疫沉淀
低非特异性结合—稳定、预封闭的磁珠以及特异性抗体尽可能减少了 HA 标签 IP 或 co-IP 实验的脱靶结合
通用—微珠兼容手动和自动工作流程(例如,Thermo Scientific KingFisher 仪器)

该封闭的磁珠表面包被着抗 HA 抗体,一种高度特异性小鼠 IgG1 单克隆抗体,可识别来源于人流感血凝素 (HA) 蛋白 的 HA 抗原决定簇标签 (YPYDVPDYA)。Pierce 抗 HA 磁珠可与磁力架配合用于手动操作,亦可用于自动化平台,如 Thermo Scientific KingFisher 仪器,以用于高通量工作流程。

规格:
产品详情:
Pierce 抗 HA 磁珠很便利,可用于重组 HA 标签蛋白的免疫沉淀 (IP) 以及其相互作用蛋白的免疫共沉淀 (co-IP)。将微珠与含有 HA 标签蛋白的细胞裂解物一起孵育,并捕获融合蛋白。随后,洗涤微珠,然后使用 0.1 M 甘氨酸(pH 值 2.0)、50 mM NaOH 或 SDS-PAGE 样品缓冲液洗脱靶蛋白。如果需要更温和的洗脱条件,可使用 2 mg/mL Pierce HA 肽。针对上述每个条件对方案进行了优化。抗 HA 抗体可用于通过 Western 印迹分析检测 HA 标签蛋白。
仅供科研使用。不可用于诊断程序。

Thermo Scientific™ Pierce 抗 HA 磁珠很便利,可用于重组 HA–标签蛋白的免疫沉淀 (IP) 以及其相互作用蛋白的免疫共沉淀 (co–IP)。将微珠与含有 HA–标签蛋白的细胞裂解物一起孵育,并捕获融合蛋白。随后,洗涤微珠,然后使用 0.1 M 甘氨酸(pH 值 2.0)、50 mM NaOH 或 SDS–PAGE 样品缓冲液洗脱靶蛋白。如果需要更温和的洗脱条件,可使用 2 mg/mL Pierce HA 肽。针对上述每个条件对方案进行了优化。抗 HA 抗体可用于通过 Western 印迹分析检测 HA–标签蛋白。

规格
最大浓度Slurry: 10mg/mL, 1% solids
配基类型抗体
蛋白质形式重组
数量5 mL
靶标HA
容量(公制)5 mL
形式液体
粒径1 μm
产品线Pierce™
类型磁珠
Unit SizeEach
内容与储存
储存在 4°C 下。

常见问题解答 (FAQ)

Are the Pierce Anti-HA Magnetic Beads (Cat. No. 88836, 88837) compatible with sodium dodecyl sulfate? If yes, up to which concentration?

Yes, the Pierce Anti-HA Magnetic Beads (Cat. No. 88836, 88837) are compatible with sodium dodecyl sulfate, but the concentration should not exceed 0.5%.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What type of recombinant protein purification resins do you offer?

We offer a variety of resins targeting His-tagged fusion proteins or GST fusion proteins. Please see this selection table to choose the right resin for your experiments. Table available at http://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-purification-isolation/protein-purification/recombinant-protein-purification.html

We also offer resins (both agarose and magnetic bead-based) with immobilized anti-c-Myc and anti-HA antibodies for targeting c-Myc and HA-tagged proteins respectively. Please check out the following links for the specific products:

-Anti-c-Myc agarose (https://www.thermofisher.com/order/catalog/product/20169?ICID=search-product)
-Anti-c Myc magnetic beads (https://www.thermofisher.com/order/catalog/product/88843?ICID=search-product)
-Anti-HA agarose (https://www.thermofisher.com/order/genome-database/antibody/HA-Epitope-Tag-Antibody-clone-2-2-2-14-Monoclonal/26181)
-Anti-HA magnetic beads: (https://www.thermofisher.com/order/catalog/product/88837?ICID=search-product)


Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.