1-Step Human Coupled IVT Kit - DNA, 40 Reaction Kit - FAQs

查看更多产品信息 1-Step Human Coupled IVT Kit - DNA - FAQs (88882, 88881)

5 个常见问题解答

体外蛋白表达系统可使用何种裂解系统？

大肠杆菌，麦胚提取物（WGE），或兔网织红细胞裂解物（RRL）都适用于体外翻译。通常情况下，RRL能够有效翻译大于30 kDa的蛋白。WGE能够兼容任何尺寸的蛋白（包括那些15–30 kDa范围内的蛋白），尽管RRL系统可能对于大蛋白的翻译更有效。我们的Expressway产品选用的是大肠杆菌。

What lysate systems can be used for in vitro protein expression?

E. coli, rabbit reticulocyte lysate (RRL), and HeLa cell lysate can all be used for in vitro translation. Our Expressway system utilizes E. coli. In general, RRL efficiently translates proteins greater than 30 kDa. The 1-Step Human In Vitro Protein Expression Kits are ideal for expressing human proteins.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

Can I use 1-Step Human Coupled IVT Kit - DNA (Cat. No. 88881, 88882) with mRNA?

This page outlines a protocols for in vitro protein expression using mRNA templates.

When comparing 1-Step Human High-Yield Mini IVT Kit with 1-Step Human Coupled IVT Kit - DNA, is one faster than the other?

No, both 1-Step Human High-Yield Mini IVT Kit and 1-Step Human Coupled IVT Kit - DNA can be run overnight (depends upon the gene).

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

Do both 1-Step Human High-Yield Mini IVT Kit and 1-Step Human Coupled IVT Kit - DNA provide the same purity of protein?

Yes, both 1-Step Human High-Yield Mini IVT Kit and 1-Step Human Coupled IVT Kit - DNA provide the same purity of protein.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.