The Thermo Scientific Pierce Glycoprotein Isolation Kit (Concanavilin A) allows isolation of glycoproteins from complex mixtures, including serum, tissue and了解更多信息
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货号
数量
89804
1 个试剂盒
货号 89804
价格(CNY)
6,496.00
Each
添加至购物车
数量:
1 个试剂盒
请求批量或定制报价
价格(CNY)
6,496.00
Each
添加至购物车
The Thermo Scientific Pierce Glycoprotein Isolation Kit (Concanavilin A) allows isolation of glycoproteins from complex mixtures, including serum, tissue and cultured cell lysates. The procedure uses concanavilin A (ConA) as a ligand to bind a specific class of glycoprotein from complex protein samples—those containing alpha-lined mannose or terminal glucose residues. This kit has been used to isolate glycoproteins from serum as well as HeLa and CHO cell lysates in approximately 50 minutes.
Features of this Glycoprotein Isolation Kit:
• High recovery—equivalent or greater glycoprotein recovery versus competitor kits and lectin resins • Fast—glycoprotein purification in less than an hour • Versatile—isolate glycoproteins from various sample types, e.g., human serum and cell lysate. • Robust—lectin does not leach from resin when processing sample • Convenient—complete kit offering lectin resins and spin columns with all necessary reagents • Compatible with Bradford-based protein assays—dialysis or protein precipitation of recovered glycoproteins is not required prior to protein assay
足够用于:从 10 份分别高达 640 µL(1 至 1.5 mg 总蛋白)的样品中分离对 ConA 具有强亲和力的糖蛋白 • ConA 外源凝集素树脂,1.1 mL • 结合/洗涤缓冲液 (5X),6.5 mL • 洗脱缓冲液,5 mL • 离心柱,10 个柱 • 收集管,20 个管
在 4°C 下储存。
常见问题解答 (FAQ)
What is the difference between your Pierce Glycoprotein Isolation Kit, ConA and WGA?
The ConA kit (Cat. No. 89804), which has concanavalin A immobilized on the resin, recognizes alpha-linked mannose, and to a lesser extent, terminal glucose residues. The WGA kit (Cat. No. 89805), which has wheat germ agglutinin immobilized on the resin, recognizes N-acetyl glucosamine (GlcNAc) groups and sialic acid.
Can I isolate my proteins based on their post-translational modification (PTM) or active binding site?
Enrichment of specific proteins or protein complexes can most easily be accomplished by using target-specific immobilized antibody or PTM specific affinity binding. We offer several kits targeting phosphorylated proteins, alpha- linked mannose and terminal glucose residues, N-acetyl glucosamine, polyubiquinated proteins, ATP binding sites, and active serine hydrolase enzymes. Choose the right protein enrichment kit for your application using the following link (http://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-purification-isolation/protein-purification/protein-enrichment.html).