Lectin affinity as an approach to the proteomic analysis of membrane glycoproteins.
AuthorsGhosh D, Krokhin O, Antonovici M, Ens W, Standing KG, Beavis RC, Wilkins JA
JournalJ Proteome Res
PubMed ID15359739
The aim was to determine the proportion of membrane glycoproteins captured using concanavalin A or wheat germ agglutinin lectin affinity chromatography. Digests of the isolated proteins were separated by reversed-phase liquid chromatography and analyzed by matrix-assisted laser desorption tandem mass spectrometry. The two lectins identified different groups of proteins with ... More
Identification of Potential Glycoprotein Biomarkers in Estrogen Receptor Positive (ER+) and Negative (ER-) Human Breast Cancer Tissues by LC-LTQ/FT-ICR Mass Spectrometry.
AuthorsSemaan SM, Wang X, Marshall AG, Sang QX
JournalJ Cancer
PubMed ID22773931
Breast cancer is the second most fatal cancer in American women. To increase the life expectancy of patients with breast cancer new diagnostic and prognostic biomarkers and drug targets must be identified. A change in the glycosylation on a glycoprotein often causes a change in the function of that glycoprotein; ... More
GlycoSuiteDB: a new curated relational database of glycoprotein glycan structures and their biological sources.
AuthorsCooper CA, Harrison MJ, Wilkins MR, Packer NH
JournalNucleic Acids Res
PubMed ID11125129
GlycoSuiteDB is a relational database that curates information from the scientific literature on glyco-protein derived glycan structures, their biological sources, the references in which the glycan was described and the methods used to determine the glycan structure. To date, the database includes most published O:-linked oligosaccharides from the last 50 ... More