Controlling selectivity of modular microbial biosynthesis of butyryl-CoA-derived designer esters.
AuthorsLee JW,Trinh CT
JournalMetabolic engineering
PubMed ID34883244
Short-chain esters have broad utility as flavors, fragrances, solvents, and biofuels. Controlling selectivity of ester microbial biosynthesis has been an outstanding metabolic engineering problem. In this study, we enabled the de novo fermentative microbial biosynthesis of butyryl-CoA-derived designer esters (e.g., butyl acetate, ethyl butyrate, butyl butyrate) in Escherichia coli with ... More
A truncated isoform of Connexin43 caps actin to organize forward delivery of full-length Connexin43
AuthorsBaum R, Nguyen VD, Maalouf M, Shimura D, Waghalter M, Srapyan S, Jin Q, Kuzmanovich L, Gaffney AT, Bell BR, Xiao S, Palatinus JA, Kléber AG, Grintsevich EE, Hong T, Shaw RM.
JournalJ Cell Biol
PubMed ID39737876
While membrane proteins such as ion channels continuously turn over and require replacement, the mechanisms of specificity of efficient channel delivery to appropriate membrane subdomains remain poorly understood. GJA1-20k is a truncated Connexin43 (Cx43) isoform arising from translation initiating at an internal start codon within the same parent GJA1 mRNA ... More
Single mutation at a highly conserved region of chloramphenicol acetyltransferase enables isobutyl acetate production directly from cellulose by Clostridium thermocellum at elevated temperatures.
AuthorsSeo H,Lee JW,Garcia S,Trinh CT
JournalBiotechnology for biofuels
PubMed ID31636704
BACKGROUND: Esters are versatile chemicals and potential drop-in biofuels. To develop a sustainable production platform, microbial ester biosynthesis using alcohol acetyltransferases (AATs) has been studied for decades. Volatility of esters endows high-temperature fermentation with advantageous downstream product separation. However, due to the limited thermostability of AATs known, the ester biosynthesis ... More
Efficient engineering of human and mouse primary cells using peptide-assisted genome editing.
Simple, efficient and well-tolerated delivery of CRISPR genome editing systems into primary cells remains a major challenge. Here we describe an engineered Peptide-Assisted Genome Editing (PAGE) CRISPR-Cas system for rapid and robust editing of primary cells with minimal toxicity. The PAGE system requires only a 30-min incubation with a cell-penetrating ... More