Lysosome Enrichment Kit for Tissues and Cultured Cells - FAQs

We recommend using 8 mL open top ultracentrifuge tubes that are compatible with the specific rotor being used in the lab. Specifically, our R&D team has used Thermo Scientific 03020 tubes (https://www.fishersci.com/shop/products/03020/03020) with Thermo Scientific Sorvall MTX 150 Micro-Ultracentrifuge (Cat. No. 46960) and S58-A Fixed Angle Rotor (Cat. No. 45866). For any other ultracentrifuge, the tubes will need to match the rotor. If the rotor does not take 8 mL tubes, the protocol can be scaled to match the tube size.

Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.

We have not tested the isolation of lysosomes from frozen tissues using this kit. We recommend using fresh tissues only as freeze-thaw compromises membrane integrity and it may affect the protein yield.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

The Lysosome Enrichment Kit for Tissues and Cultured Cells is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Lysosome Enrichment Kit for Tissues and Cultured Cells has been designed for isolation and enrichment of intact lysomes from crude cell and tissue extracts. We do not have any guidelines for isolation of the cytosolic fraction as the rest of the density gradient was not defined in-house.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

We offer the following kits for cell fractionation:

Mem-PER Plus Membrane Protein Extraction Kit, Cat. No. 89842
NE-PER Nuclear and Cytoplasmic Extraction Reagents, Cat. No. 78835
Subcellular Protein Fractionation Kit for Cultured Cells, Cat. No. 78840
Subcellular Protein Fractionation Kit for Tissues, Cat. No. 87790
Mitochondrial Isolation Kit for Cultured Cells, Cat. No. 89874
Mitochondrial Isolation kit for Tissue, Cat. No. 89801
Lysosome Enrichment Kit for Tissues and Cultured Cells, Cat. No. 89839

Find additional tips, troubleshooting help, and resources within our Cell Lysis and Fractionation Support Center.

Isolation of subcellular fractions and concentration of low-abundantce proteins allows for more efficient identification and study of the proteins of interest.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.