Mem-PER™ Plus 膜蛋白提取试剂盒
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Mem-PER™ Plus 膜蛋白提取试剂盒
Thermo Scientific™

Mem-PER™ Plus 膜蛋白提取试剂盒

Thermo Scientific Mem-PER Plus 膜蛋白提取试剂盒可通过基于试剂的简单程序实现整合膜蛋白和膜相关蛋白的小规模增溶和富集。Mem-PER Plus 试剂盒的特点:•提取和分离—交叉污染率极低(通常低于 10%了解更多信息
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货号数量
898421 个试剂盒
货号 89842
价格(CNY)
3,172.00
飞享价
Ends: 31-Dec-2025
4,262.00
共减 1,090.00 (26%)
Each
添加至购物车
数量:
1 个试剂盒
请求批量或定制报价
价格(CNY)
3,172.00
飞享价
Ends: 31-Dec-2025
4,262.00
共减 1,090.00 (26%)
Each
添加至购物车
Thermo Scientific Mem-PER Plus 膜蛋白提取试剂盒可通过基于试剂的简单程序实现整合膜蛋白和膜相关蛋白的小规模增溶和富集。

Mem-PER Plus 试剂盒的特点:

提取和分离—交叉污染率极低(通常低于 10%),膜蛋白组分中仅会掺入极少量细胞溶质蛋白
细胞或组织—能从培养的哺乳动物细胞和哺乳动物组织中进行高效提取
下游兼容性—通过 SDS-PAGE、Western 印迹、免疫沉淀和蛋白检测分析膜蛋白提取物
快速简单—可在约 1 小时内分离出膜蛋白
不需要特殊设备—只需要台式微量离心机、管、均质仪和移液器

Mem-PER Plus 试剂盒可使用简单的台式微量离心程序从培养细胞和组织中有效分离膜蛋白。它可以有效溶解含有一个或两个跨膜结构域的整合膜蛋白,并将它与细胞溶质蛋白分离开。本试剂盒可以从培养的哺乳动物细胞以及硬质和软质哺乳动物组织中提取和选择性富集整合膜蛋白和膜联蛋白。与原始的 Mem-PER 试剂盒相比,该 Mem-PER Plus 试剂盒具备很多优点。方案更简单,组分可直接兼容许多下游应用,如 SDS-PAGE、Western 印迹、BCA、免疫沉淀以及胺反应性蛋白标记技术。

包括:
试剂盒含有细胞洗涤液、透化缓冲液和增溶缓冲液。

传统膜蛋白分离方法既繁琐又费时,而且需要梯度分离和昂贵的超速离心设备。此 Mem-PER Plus 膜蛋白提取试剂盒可利用选择性提取方案从培养的哺乳动物细胞或组织中富集整合膜蛋白和膜联蛋白,所用试剂以温和的去污剂为主。传统分离方法通常需要依据疏水性进行分离,而选择性去污剂提取方案可以省去这一繁琐的相分离过程,从而提高了实验的重现性和处理通量。

先使用温和的去污剂对细胞进行透膜处理,使细胞释放可溶性细胞溶质蛋白,然后再使用第二种去污剂溶解膜蛋白。提取效率和得率取决于细胞类型以及整合膜蛋白在脂质双分子层上的跨膜次数。对于含有一个或两个跨膜结构域的膜蛋白,提取效率通常可达到 90%。仅有极少细胞溶质蛋白会掺入膜蛋白组分中,因此交叉污染率通常不足 10%。

更多产品数据
高效哺乳动物膜蛋白提取

相关产品
用于培养细胞的亚细胞蛋白分离试剂盒
用于组织的亚细胞蛋白分离试剂盒
台式离心机
仅供科研使用。不可用于诊断程序。
规格
数量1 个试剂盒
试剂类型细胞裂解缓冲液、去污剂溶液、亚细胞分离
足够用于50 份样品
形式液体
产品线Mem-PER™
产品类型蛋白提取试剂盒
Unit SizeEach
内容与储存
接收后,在 4°C 下储存细胞洗涤液和增溶缓冲液。将透化缓冲液等分并储存于 -20°C 下,避免冻融。将产品置于冰上运输。

常见问题解答 (FAQ)

I am using the Mem-PER Plus Membrane Protein Extraction Kit with less than 5 X 10E6 cells. Should I scale down the amount of reagents accordingly?

The kit reagents can be scaled up or down as long as the ratio of reagents to cells is maintained. However, we don't have specific guidelines for the range of cell numbers that can be used because protein yields can vary depending on the cell line. It is important to note that the process of scaling the kit reagents up or down and determining the appropriate cell numbers for optimal protein yield will require empirical optimization by the investigator.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What kind of detergent does the Permeabilization Buffer from the Mem-PER Plus Membrane Protein Extraction Kit (Cat. No 89842) contain?

The composition of the Permeabilization Buffer, including the identity and concentration of any detergent therein, is proprietary. However, the Permeabilization Buffer contains a very small amount of a weak non-ionic detergent to facilitate the permeabilization of the cells and release of the cytosolic proteins.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center

Will the Mem-PER Plus Membrane Protein Extraction Kit (Cat. No. 89842) extract membrane proteins from the plasma membrane only or also from other intra-cellular membranes (e.g., nuclear, mitochondrial, endoplasmic reticulum)?

The Permeabilization buffer in the Mem-PER Plus Membrane Protein Extraction Kit (Cat. No. 89842) creates pores in the plasma membrane of cells that releases cytosolic proteins. The permeabilized cells are centrifuged, and the supernatant contains the cytosolic protein fraction. The pellet is then resuspended in the Solubilization buffer containing a proprietary detergent that solubilizes plasma, mitochondrial, and ER membrane proteins. Subsequent centrifugation yields a supernatant containing solubilized membrane and membrane-associated proteins.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center

Can microalgae be processed with the Mem-PER Plus Membrane Protein Extraction Kit (Cat. No. 89842)?

The Mem-PER Plus Membrane Protein Extraction Kit is not suitable for any organism that has a cell wall, including algae, plants, yeast, or bacteria.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Will M-PER Mammalian Protein Extraction Reagent extract membrane or cytoskeletal proteins?

M-PER Mammalian Protein Extraction Reagent can extract some membrane or cytoskeletal proteins, but the extraction efficiency is not consistent. The reagent was not intended to specifically extract these proteins. We recommend using Mem-PER Plus Membrane Protein Extraction Kit (Cat. No. 89842) for membrane protein extraction or Subcellular Protein Fractionation Kit for Cultured Cells (Cat. No. 78840) for compartmental extraction.

Find additional tips, troubleshooting help, and resources within our Cell Lysis and Fractionation Support Center.