Pierce™ 多肽脱盐离心柱
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Pierce™ 多肽脱盐离心柱
Thermo Scientific™

Pierce™ 多肽脱盐离心柱

Thermo Scientific Pierce 多肽脱盐离心柱是即用型离心柱，能够在酶消化后对多肽样品进行高效脱盐。每根色谱柱所含的聚合物基疏水树脂可为肽样品提供了优异的结合和回收特性，以便用于质谱分析等方法的制备。Pierce 多肽脱盐离心柱的特点包括：•去除 MS 干扰污染物—可显著减少信号抑制并提高质谱的灵敏度• 高结合能力—特殊疏水聚合物基树脂可实现从了解更多信息
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货号数量
8985225柱
8985150柱
货号 89852
价格（CNY)
2,650.00
飞享价
Ends: 31-Dec-2025
3,271.00
共减 621.00 (19%)
Each
添加至购物车
数量:
25柱
价格（CNY)
2,650.00
飞享价
Ends: 31-Dec-2025
3,271.00
共减 621.00 (19%)
Each
添加至购物车
Thermo Scientific Pierce 多肽脱盐离心柱是即用型离心柱，能够在酶消化后对多肽样品进行高效脱盐。每根色谱柱所含的聚合物基疏水树脂可为肽样品提供了优异的结合和回收特性，以便用于质谱分析等方法的制备。

Pierce 多肽脱盐离心柱的特点包括：
•去除 MS 干扰污染物—可显著减少信号抑制并提高质谱的灵敏度
• 高结合能力—特殊疏水聚合物基树脂可实现从 5 μg 到 5 mg 的天然多肽和 TMT 标记多肽的出色肽上样回收率
• 易于使用—树脂以一次性离心柱形式提供，适用于多种常见的 2 mL 试管
• 兼容性—树脂经过与多种复杂样品（包括 TMT 标记多肽）的验证，可在极端 pH 条件下保持稳定

Pierce 多肽脱盐离心柱提供了一种方便，可重现性好的方法来在质谱分析之前对多肽样品进行脱盐和去除污染物。每个离心柱都可以结合 5 μg 到 5 mg 的天然多肽和 TMT 标记多肽。离心柱形式允许在约 30 分钟内同时处理多个样品（每份样品 10 至 300 μL）。

质谱分析对于研究生物化合物至关重要。然而，在样品制备过程中使用的许多缓冲液和试剂将干扰质谱分析，导致质谱质量差，灵敏度低。Pierce 多肽脱盐离心柱旨在以较少的样品损失去除多种干扰亲水性样品的污染物，从而通过质谱法对经过消化的蛋白质样品进行灵敏而全面的分析。

For Research Use Only. Not for use in diagnostic procedures.
规格
最终产品类型肽段
数量25柱
运输条件室温
检测方法质谱分析
产品规格离心柱
产品线Pierce™
产品类型脱盐离心柱
Unit SizeEach
内容与储存
储存在 2–8°C 下。

常见问题解答 (FAQ)

How do I determine if my sample is compatible and ready for LC-MS?

Samples prepared using LC/MS grade reagents are suitable for LC-MS; however, particulates and other small molecules can all interfere with liquid chromatography separation and mass spectrometer source ionization. We recommend visual inspection of samples for particulate matter and using Pierce Peptide Desalting Spin Columns (Cat. Nos. 89851, 89852), Pierce C18 Spin Tips (Cat. No. 84850) or an in-line C18 trap column (https://www.thermofisher.com/order/catalog/product/164564-CMD) to remove non-volatile salts before MS analysis.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

After TMT reagent labeling, I still see a lot of excess TMT tags in my sample. How should I remove it?

We offer EasyPep MS sample preparation kits (EasyPep Maxi Sample Prep Kit (Cat. No. A45734), EasyPep Mini MS Sample Prep Kit (Cat. No. A40006), EasyPep 96 MS Sample Prep Kit (Cat. No. A45733)) that contain wash buffers specifically formulated to clean up TMT-labeled protein digests. To remove excess TMT reagent from samples prepared using other sample preparation methods, we recommend using Pierce Peptide Desalting Spin Columns (Cat. Nos. 89851, 89852) with extra washes using 5% methanol. The Pierce High pH Reversed-Phase Peptide Fractionation Kit (Cat. No. 84868) can also be used to remove excess unreacted TMT tags before collecting fractions.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

I'm seeing a peak in my mass spectrometry analysis results that seems to always be present in my system. What can I do?

The peak most likely represents PEG or a similar contaminant. Clean your MS system and/or perform sample clean-up using Pierce Peptide Desalting Spin Columns (Cat. Nos. 89851, 89852) or Pierce C18 Resin. In addition, ensure that LC-MS pre-blended solvents are being used (formic acid/water, formic acid/acetonitrile, etc.)

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

I cleaned up my peptide sample using C18 or desalting columns, but I lost most of my peptides. What went wrong?

Peptides do not bind well to reversed phase resins at neutral pH or in the presence of organic solvents (e.g., acetonitrile). Acidify protein digest samples using formic acid or trifluoroacetic acid (TFA) to pH <3 before desalting. Ensure that samples do not contain organic solvents before and after clean-up by drying them down using a SpeedVac concentrator or equivalent.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

If I already removed small contaminants and detergents at the protein level, do I still need to desalt my peptides prior to mass spectrometry analysis?

Yes. We recommend performing additional cleanup after protein digestion to remove any residual salts or partially digested proteins using Pierce Peptide Desalting Spin Columns (Cat. Nos. 89851, 89852), Pierce C18 Spin Tips (Cat. No. 84850) or an in-line C18 trap column (https://www.thermofisher.com/order/catalog/product/164564-CMD).

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

View all Pierce™ 多肽脱盐离心柱 FAQs