足够用于:800 cm2 膜(10 微型凝胶印迹) • 稳定链霉素亲和素-HRP,1.5 mL • 鲁米诺/增强剂溶液,80 mL • 稳定的过氧化氢溶液,80 mL • 封闭缓冲液(产品编号 89880A),500 mL • 洗涤缓冲液 (4X),500 mL • 底物平衡缓冲液,500 mL
在 4°C 下储存。
常见问题解答 (FAQ)
SuperSignal West Pico化学发光底物是否适用于核酸印迹(Southern blotting等)?
< p >是的。然而,SuperSignal West Pico化学发光的底物是为Western blots优化的,通常对大多数核酸操作不够敏感。对于Southern和Northern blotting, 使用我们的North2South化学发光核酸杂交和检测试剂盒(货号17097),用于HRP的North2South化学发光底物(货号17097)或者如果用生物素化探针杂交的话,使用化学发光核酸检测试剂盒(货号 89880)。< / p >
SuperSignal West Femto化学发光底物是否适用于核酸印迹(Southern blotting等)?
< p >是的。然而,SuperSignal West Femto化学发光的底物是为Western blots优化的,通常对大多数核酸操作不够敏感。对于Southern和Northern blotting, 使用我们的North2South化学发光核酸杂交和检测试剂盒(货号17097),用于HRP的North2South化学发光底物(货号17097)或者如果用生物素化探针杂交的话,使用化学发光核酸检测试剂盒(货号 89880)。< / p >
SuperSignal West Dura化学发光底物是否适用于核酸印迹(Southern blotting等)?
< p >是的。然而,SuperSignal West Dura化学发光的底物是为Western blots优化的,通常对大多数核酸操作不够敏感。对于Southern和Northern blotting, 使用我们的North2South化学发光核酸杂交和检测试剂盒(货号17097),用于HRP的North2South化学发光底物(货号17097)或者如果用生物素化探针杂交的话,使用化学发光核酸检测试剂盒(货号 89880)。< / p >
Will SuperSignal West Pico PLUS Chemiluminescent Substrate work with nucleic acid blots (Southern blotting, etc.)?
Yes. However, SuperSignal West Pico PLUS Chemiluminescent Substrate was optimized for use in Western blots and is generally not sensitive enough for most nucleic acid protocols. For Southern and Northern blotting, use our North2South Chemiluminescent Nucleic Acid Hybridization and Detection Kit (Cat. No. 17097), North2South Chemiluminescent Substrate for HRP (Cat. No. 17295), or the Chemiluminescent Nucleic Acid Detection Kit, if probing for biotinylated probes (Cat. No. 89880).
Will SuperSignal West Femto Maximum Sensitivity Substrate work with nucleic acid blots (Southern blotting, etc.)?
Yes. However, SuperSignal West Femto Maximum Sensitivity Substrate was optimized for use in Western blot sand is generally not sensitive enough for most nucleic acid protocols. For Southern and Northern blotting, use our North2South Chemiluminescent Nucleic Acid Hybridization and Detection Kit (Cat. No. 17097), North2South Chemiluminescent Substrate for HRP (Cat. No. 17295), or the Chemiluminescent Nucleic Acid Detection Kit, if probing for biotinylated probes (Cat. No. 89880).