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Zeba™ Spin Desalting Columns, 7K MWCO, 0.5 mL, 50 Columns - FAQs

Zeba™ Spin Desalting Columns, 7K MWCO, 0.5 mL, 50 Columns - FAQs

查看更多产品信息 Zeba™ Spin Desalting Columns, Plates, and Cartridges, 7K MWCO, 0.5–100 mL - FAQs (89891, 89890, A40002469, 89808, A40002475, 89807, 89935, 89934, 89878, 89889, 89877, 89894, 89883, 89893, 89882, 89892)

15 个常见问题解答

What reagents and conditions are Zeba Desalting columns, plates, and cartridges compatible with?

Zeba Desalting columns, plates, and cartridges are compatible with a range of reagents and conditions. They have been confirmed to be compatible with the following:
- pH range of 3-10
- Mild oxidants/reductants
- Chaotropes (stable in 8 M urea and 6 M guanidine HCl)
- Salts
- Alcohols up to 20%
- Organic solvents such as dimethyl sulfoxide (DMSO) and dimethylformamide (DMF) (we recommend using a step-gradient of increasing concentration of the organic solvent during column equilibration)

Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.

What product do you recommend for removing sodium azide from the Alix Monoclonal Antibody (3A9) (Cat. No. MA1-83977) storage solution?

We recommend using Zeba Spin Desalting Columns, Plates, and Cartridges (Cat. No. 89882, 89883) to remove sodium azide from the Alix Monoclonal Antibody (3A9) storage solution.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Can you provide the shelf life for Zeba Spin Desalting columns?

Zeba Spin Desalting columns are covered under our general 1-year warranty and are guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf).

Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.

With Zeba Spin Desalting columns, plates, and cartridges, how can I completely get rid of glycerol and detergent from my sample?

Glycerol and sugars add viscosity to a sample, while detergents create micelles, making the removal of each difficult. Although other methods are a better choice for removing these components, Zeba columns can be used by modifying the desalting protocol. Modifications include diluting the sample, processing a smaller volume of the sample, and adding the sample slowly to allow it to enter the gel and equilibrate into the pores before processing.

Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.

After desalting with your Zeba Spin Desalting columns, I still have leftover salt in my sample. What should I do to get rid of all the salt?

You can either reduce the volume of sample processed in each column (the product manual indicates the volume range recommended for each column size) or run the flow-through from one column over a fresh column.

Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.

Can Zeba Spin Desalting columns, plates, and cartridges be used for glycerol and detergent removal?

Glycerol and sugars add viscosity to a sample, while detergents create micelles, making the removal of each difficult. Although other methods are a better choice for removing these components, Zeba columns can be used by modifying the desalting protocol. Modifications include diluting the sample, processing a smaller volume of the sample, and adding the sample slowly to allow it to enter the gel and equilibrate into the pores before processing.

Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.

Is my sample likely to become diluted following desalting with Zeba Spin Desalting columns and plates?

Centrifugation-based desalting results in minimal dilution. A slight dilution of the protein solution results if the optional stacker buffer is added that ensures maximum protein recovery.

When used properly, the volume recovered from the column equals the volume of sample (and buffer) added to the column.

Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.

Can I remove DyLight, Alexa Fluor dye or other dyes using Zeba Spin Desalting columns, plates, and cartridges?

No, excess amounts of DyLight dyes (and any other planar molecule with multiple rings in their structure) “act” larger than their stated molecular weights in desalting columns. We do not recommend desalting, but refer researchers to our Pierce Dye Removal Columns (Cat. No. 22858) or Zeba Dye and Biotin Removal Spin Columns and Filter Plates (Cat. No. A44296) for this purpose.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Can I use Zeba Spin Desalting columns, plates, and cartridges for recovery of nucleic acids?

Yes, for example, the 7K Zeba columns have good recovery of dsDNA ladder down to 10 bp.

Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.

Are Zeba Spin Desalting columns, plates, and cartridges compatible with salts/organics?

Yes, Zeba Spin Desalting columns, plates, and cartridges are compatible with most salts. The resin is stable to some organics. As organics may affect performance, we suggest using less than 10% organics.

Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.

How do your Zeba Spin Desalting columns and plates work?

A centrifuge is used to first remove the resin's void volume of liquid, followed by sample addition and centrifugation. After centrifugation, the macromolecules in the sample have moved through the column in approximately the same initial volume, but the small molecules have been forced into the pores of the resin and replaced by the buffer that was used to pre-equilibrate the gel-filtration matrix. Spin formats eliminate the need to wait for samples to emerge by gravity flow and require no chromatography system, allowing for multiple-sample processing simultaneously.

Note: The addition of larger volumes of buffer or longer centrifugation times than listed in the protocol will result in smaller molecules eventually emerging from the desalting column.

Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.

Note: The addition of larger volumes of buffer or longer centrifugation times than listed in the protocol will result in smaller molecules eventually emerging from the desalting column.

Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.

What is desalting?

Desalting is the process where porous particles or beads are used to separate molecules of different sizes (known as gel filtration or size exclusion). Small molecules enter into the pores in the resin, resulting in a longer path length through the desalting column when compared to large molecules which pass in the space between the beads This increased path length for molecules below the MWCO allows the separation of small and large molecules.

Our desalting resins are rated by what MWCO molecules can pass through. They also have a lower MWCO (1K or 2K) that can be effectively separated from larger molecules. Molecules in between these values cannot be effectively separated from larger or smaller molecules, given our protocols.

Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.