Shatavari supplementation in postmenopausal women alters the skeletal muscle proteome and pathways involved in training adaptation.
AuthorsO'Leary MF,Jackman SR,Bowtell JL
JournalEuropean journal of nutrition
PubMed ID38214710
PURPOSE: Shatavari is an understudied, widely available herbal supplement. It contains steroidal saponins and phytoestrogens. We previously showed that six weeks of shatavari supplementation improved handgrip strength and increased markers of myosin contractile function. Mechanistic insights into shatavari's actions are limited. Therefore, we performed proteomics on vastus lateralis (VL) samples ... More
Autoreactive T lymphocytes crossing the blood-brain barrier (BBB) into the central nervous system (CNS) play a crucial role in the initiation of demyelination and neurodegeneration in multiple sclerosis (MS). Recently, extracellular vesicles (EV) secreted by BBB endothelial cells (BBB-EC) have emerged as a unique form of cell-to-cell communication that contributes ... More
Optimization of a Protocol for Protein Extraction from Calcified Aortic Valves for Proteomics Applications: Development of a Standard Operating Procedure.
The comprehension of the pathophysiological mechanisms, the identification of druggable targets, and putative biomarkers for aortic valve stenosis can be pursued through holistic approaches such as proteomics. However, tissue homogenization and protein extraction are made difficult by tissue calcification. The reproducibility of proteome studies is key in clinical translation of ... More
TGFB1-induced extracellular expression of TGFBIp and inhibition of TGFBIp expression by RNA interference in a human corneal epithelial cell line.
AuthorsYellore VS, Rayner SA, Aldave AJ
JournalInvest Ophthalmol Vis Sci
PubMed ID20881301
'To report the increased production of extracellular transforming growth factor ß-induced protein (TGFBIp) by human corneal epithelial cells (HCECs) after induction by TGFB1 and the inhibition of TGFBIp production in induced and noninduced HCECs by RNA interference (RNAi).' ... More
Repair of full-thickness femoral condyle cartilage defects using allogeneic synovial cell-engineered tissue constructs.
AuthorsPei M, He F, Boyce BM, Kish VL
JournalOsteoarthritis Cartilage
PubMed ID19128988
Synovium-derived stem cells (SDSCs) have proven to be superior in cartilage regeneration compared with other sources of mesenchymal stem cells. We hypothesized that conventionally passaged SDSCs can be engineered in vitro into cartilage tissue constructs and the engineered premature tissue can be implanted to repair allogeneic full-thickness femoral condyle cartilage ... More
Adult hippocampal neural stem and progenitor cells regulate the neurogenic niche by secreting VEGF.
AuthorsKirby ED, Kuwahara AA, Messer RL, Wyss-Coray T
Journal
PubMed ID25775598
The adult hippocampus hosts a population of neural stem and progenitor cells (NSPCs) that proliferates throughout the mammalian life span. To date, the new neurons derived from NSPCs have been the primary measure of their functional relevance. However, recent studies show that undifferentiated cells may shape their environment through secreted ... More
Trehalose, an mTOR-Independent Inducer of Autophagy, Inhibits Human Cytomegalovirus Infection in Multiple Cell Types.
AuthorsBelzile JP, Sabalza M, Craig M, Clark E, Morello CS, Spector DH
JournalJ Virol
PubMed ID26559848
Human cytomegalovirus (HCMV) is the major viral cause of birth defects and a serious problem in immunocompromised individuals and has been associated with atherosclerosis. Previous studies have shown that the induction of autophagy can inhibit the replication of several different types of DNA and RNA viruses. The goal of the ... More