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View additional product information for Zeba™ Spin Desalting Columns, Plates, and Cartridges, 7K MWCO, 0.5–100 mL - FAQs (89891, 89890, A40002469, 89808, A40002475, 89807, 89935, 89934, 89878, 89889, 89877, 89894, 89883, 89893, 89882, 89892)
11 product FAQs found
Yes, Zeba Desalting columns, plates, and cartridges can be autoclaved at 115 degrees C for 30 min.
Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.
Zeba Desalting columns, plates, and cartridges are compatible with a range of reagents and conditions. They have been confirmed to be compatible with the following:
- pH range of 3-10
- Mild oxidants/reductants
- Chaotropes (stable in 8 M urea and 6 M guanidine HCl)
- Salts
- Alcohols up to 20%
- Organic solvents such as dimethyl sulfoxide (DMSO) and dimethylformamide (DMF) (we recommend using a step-gradient of increasing concentration of the organic solvent during column equilibration)
Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.
Yes, Zeba Desalting Chromatography Cartridges can be regenerated and stored for later use. The catridges may be reused multiple times without significant loss of performance.
For regeneration, wash the resin with 3-5 column volumes of water or buffer before applying the next sample. For storage, wash the resin with 5 column volumes of 0.02-0.05% sodium azide in water. Attach the supplied bottom cap and then the top plug and store the cartridge at 4 degrees C.
Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.
We recommend storing Zeba Spin Desalting Columns, Plates, and Cartridges at 4 degrees C.
Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.
We recommend using Zeba Spin Desalting Columns, Plates, and Cartridges (Cat. No. 89882, 89883) to remove sodium azide from the Alix Monoclonal Antibody (3A9) storage solution.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
Glycerol and sugars add viscosity to a sample, while detergents create micelles, making the removal of each difficult. Although other methods are a better choice for removing these components, Zeba columns can be used by modifying the desalting protocol. Modifications include diluting the sample, processing a smaller volume of the sample, and adding the sample slowly to allow it to enter the gel and equilibrate into the pores before processing.
Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.
Glycerol and sugars add viscosity to a sample, while detergents create micelles, making the removal of each difficult. Although other methods are a better choice for removing these components, Zeba columns can be used by modifying the desalting protocol. Modifications include diluting the sample, processing a smaller volume of the sample, and adding the sample slowly to allow it to enter the gel and equilibrate into the pores before processing.
Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.
No, excess amounts of DyLight dyes (and any other planar molecule with multiple rings in their structure) “act” larger than their stated molecular weights in desalting columns. We do not recommend desalting, but refer researchers to our Pierce Dye Removal Columns (Cat. No. 22858) or Zeba Dye and Biotin Removal Spin Columns and Filter Plates (Cat. No. A44296) for this purpose.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
Yes, for example, the 7K Zeba columns have good recovery of dsDNA ladder down to 10 bp.
Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.
Yes, Zeba Spin Desalting columns, plates, and cartridges are compatible with most salts. The resin is stable to some organics. As organics may affect performance, we suggest using less than 10% organics.
Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.
Desalting is the process where porous particles or beads are used to separate molecules of different sizes (known as gel filtration or size exclusion). Small molecules enter into the pores in the resin, resulting in a longer path length through the desalting column when compared to large molecules which pass in the space between the beads This increased path length for molecules below the MWCO allows the separation of small and large molecules.
Our desalting resins are rated by what MWCO molecules can pass through. They also have a lower MWCO (1K or 2K) that can be effectively separated from larger molecules. Molecules in between these values cannot be effectively separated from larger or smaller molecules, given our protocols.
Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.