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查看更多产品信息 GeneChip™ Resequencing Assay Kit - FAQs (900447)
5 个常见问题解答
The Human Mitochondrial Resequencing Array Library files can be installed as follows:
- Go to the product page: https://www.thermofisher.com/order/catalog/product/900886?SID=srch-srp-900886
- Click on Technical Documents
- Download the library files to the local computer
- Open the AGCC Library file installer
- In AGCC Library file installer choose: Import parameters from a GCOS library file package CD or folder
- Click Next
- In the Select CD drive or folder location of source files to import: click on Browse and navigate to location of Library file that was just downloaded. Do not change the Save location
- Click Next.
- The library files available to install will be listed.
- Choose the Mitochip_2
- Click Start.
- The software will prompt that the files were successfully imported.
- Close the Library file importer. The Library files are now installed.
Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.
In general the arrays and software are most optimal for detection of SNPs. Unknown indels will be very difficult to automatically detect with the current array and software. For known indels we can try to tile (see below) for them but do not make any claims with respect to accuracy.
Indels: Known insertions can be tiled for.
Specific performance is not guaranteed.: Known homozygous deletions can be tiled for.
Specific performance is not guaranteed.: Unknown INDELS or heterogeneous deletions will not be detected.
Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.
Custom arrays must be ordered in full manufacturing lots. See below for lot minimums.
Format: Lot Size
49: 45 plus/minus 5 arrays
100: 90 plus/minus 5 arrays
169: 155 plus/minus 5 arrays
Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.
Three formats of custom arrays are available that allow for the analysis of approximately 300kb, 100kb and 50kb of double stranded sequences. Please refer to the CustomSeq Design Guide for details on array content considerations. The exact maximum capacities are listed below.
Format: Maximum Capacity
49: 303,366
100: 117,254
169: 47,947
Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.
The ABACUS algorithm was developed to read our early resequencing arrays in collaboration between Affymetrix and Aravinda Chakravarti's group, principally David Cutler and Michael Zwick, at Johns Hopkins University. ABACUS is available as shareware/freeware to academic researchers through JHU. No support is available for ABACUS. The principles of ABACUS were used by Affymetrix in our development of GeneChip Sequence Analysis Software (GSEQ). GSEQ 4.0 was developed by Affymetrix and includes a number of subsequent improvements and visualization tools. GSEQ 4.1 is now available through and fully supported by Affymetrix for use with the GeneChip CustomSeq Array Program.
Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.