GeneChip™ 人类基因组 U133 Plus 2.0 阵列
GeneChip™ 人类基因组 U133 Plus 2.0 阵列
Applied Biosystems™

GeneChip™ 人类基因组 U133 Plus 2.0 阵列

GeneChip™ 人类基因组 U133 Plus 2.0 阵列芯片较早、较全面的全人类基因组表达谱芯片的优势• 便捷的单一芯片视图。•人类基因组 U133了解更多信息
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货号阵列数量
90046730 阵列
货号 900467
价格(CNY)
-
阵列数量:
30 阵列
GeneChip™ 人类基因组 U133 Plus 2.0 阵列芯片

较早、较全面的全人类基因组表达谱芯片的优势
• 便捷的单一芯片视图。
•人类基因组 U133 完全覆盖 6,500 个其他基因,可分析超过 47,000 个转录本。
•使用探针组的功能,为每个转录本获取多个独立的测量结果,可为任何微阵列平台提供较高的准确性与重现性。
• GeneChip 系统:更丰富的数据、更深刻的见识、更好的决策

阵列特征
GeneChip 人类基因组 U133 集上代表的所有探针组均在 GeneChip 人类基因组 U133 Plus 2.0 阵列上相同地复制。这些探针组的来源序列选自 GenBank™、dbEST 和 RefSeq。序列簇是从 UniGene 数据库(组建 133,2001 年 4 月 20 日)创建的,然后通过分析和与其它一些公开可用的数据库(包括华盛顿大学 EST 追踪存储库和加州大学 Santa Cruz Golden Path 人类基因组数据库)进行比较来改进(发布于 2001 年 4 月)。

另外,还有 9,921 个探针组,代表约 6,500 个基因。这些基因序列从 GenBank、dbEST 和 RefSeq 中进行选择。序列簇是从 UniGene 数据库(组建 159,2003 年 1 月 25 日)创建的,通过分析和与其它一些公开可用的数据库(包括华盛顿大学 EST 追踪存储库和 NCBI 人类基因组组装(组建 31))进行比较来改进。
仅供科研使用。不可用于诊断程序。
规格
产品线GeneChip™
数量30 arrays
类型人类基因组 U133 Plus 2.0 阵列
数组转录组谱分析
产品规格阵列检测盒
阵列数量30 阵列
种属
Unit SizeEach

常见问题解答 (FAQ)

What reagent kit should I use with my array?

Please refer to the Microarray Reagent Guide for Arrays and Expression Kits to match the correct reagents your array.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

How are transcript IDs assigned for WT arrays if a gene has multiple transcript IDs?

The first assigned mRNA/gene that is listed in the annotation file for a given probe set or transcript cluster is considered the "best" assignment based on data source quality ranking and assignment scoring system. To get a single best mRNA/gene assignment you can simply take the first one in the list in the annotation file.

Sometimes, there might be multiple best hits between transcript clusters (or probe set) and a mRNA/gene, with identical score and data source quality. In that case, the transcript assignments are ranked based on their descriptions (e.g., title from the GenBank record).

For transcript clusters that detect genes that are alternatively spliced, the different spliced forms will be ranked by length with the longest ones listed first.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

Does the GeneChip miRNA 3.1 Array Strip require a different hyb/wash/stain kit than the GeneChip 3' IVT arrays and GeneChip ST array strips?

The hyb/wash/stain kit for the GeneChip miRNA 3.1 Array Strips is different than that for the 3' IVT arrays and GeneChip ST array strips. Additional wash A steps have been added to ensure adequate washing after the array strips have been stained.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

What do the suffixes mean at the end of each probeset in GeneChip 3' IVT arrays?

The probe set names never change, but they can give you an idea of what was known about the sequence at the time of design.
_at: All probes hit one known transcript.
_a: All probes in the set hit alternate transcripts from the same gene.
_s: All probes in the set hit transcripts from different genes.
_x: Some probes hit transcripts from different genes.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

Can I store my GeneChip 3' IVT Array after hybridization?

Yes, but we recommend storing your GeneChip 3' IVT Array after hybridization and prior to the fluidics step, for no longer than 3 hours at 4 degrees C. Please remove the hybridization cocktail and fill with wash A before storing.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.